高效反硝化细菌驯化培养及其包埋填料低温条件下应用性能研究  

Domestication Culture of Denitrifying Bacteria and Its Application Performance Under Low Temperature Conditions

作　　者：周亚坤 Zhou Yakun(Beijing General Municipal Engineering Design&Research Institute Co.,Ltd.,Beijing,100082)

机构地区：[1]北京市市政工程设计研究总院有限公司,北京100082

出　　处：《当代化工研究》2022年第12期1-4,共4页Modern Chemical Research

摘　　要：为有效提高冬季低温条件下市政污水厂的脱氮能力,以回流污泥为菌源进行驯化培养,得到了反硝化速率为546.34mg·(L·h)^(-1)的高效反硝化脱氮菌群,单位质量污泥的反硝化速率可达60.66mg·(g·h)^(-1),以此为菌源制作的包埋反硝化填料,针对冬季低温条件下污水厂的二级出水,可使其出水TN浓度稳定在5mg·L^(-1)以下,表现出了良好的脱氮性能。通过分子生物学检测发现,微生物菌群结构在驯化条件的影响下发生适应性改变,菌群多样性降低,但同具反硝化功能的细菌多样性得到显著提升,两种反硝化核心菌属Thauera和Pseudomonas比例由初始的2.85%提高到69.86%,最终菌群结构与驯化条件形成动态平衡,这是驯化后的细菌及其包埋填料保持高效脱氮性能的主要原因。In order to effectively improve the denitrification performance of the sewage plant under low temperature in winter,the returned sludge was domesticated and cultured,and a high-efficiency denitrification denitrification bacteria group with denitrification rate of 546.34mg·(L·h)^(-1)was obtained.The denitrification rate per unit mass of sludge can reach 60.66 mg·(g·h)^(-1).The embedded denitrification filler made from this bacteria source can stabilize the TN concentration of the secondary effluent of the sewage plant under low temperature in winter below 5 mg·L^(-1),it shows good denitrification performance.Through molecular biology detection,it is found that the structure of microbial flora changes adaptively under the influence of domestication conditions,and the diversity of flora decreases,but the diversity of bacteria with denitrification function has been significantly improved.The proportion of two denitrification core bacteria Thauera and Pseudomonas has increased from 2.85%to 69.86%.Finally,the structure of microbial flora forms a dynamic balance with domestication conditions.This is the main reason why the domesticated bacteria and their embedded fillers maintain high nitrogen removal performance.

关 键 词：广谱性反硝化细菌 间歇式 微生物群落结构 分子生物学 种群平衡 包埋反硝化填料 低温脱氮性能 

分 类 号：G712.4[文化科学—职业技术教育学]