硼酸钠-冰醋酸亚甲蓝染色法检测幽门螺旋杆菌效果评价  

Evaluation on the outcomes of detecting Helicobacter pylori sodium borate-glacial acetic acid methylene blue staining

作　　者：邓士杰 喻朝霞 DENG Shijie;YU Zhaoxia(Department of Pathology,Anqing First People′s Hospital Affiliated to Anhui Medical University,Anqing 246000,China)

机构地区：[1]安徽医科大学附属安庆市第一人民医院病理科,安徽安庆246000

出　　处：《皖南医学院学报》2022年第3期246-248,共3页Journal of Wannan Medical College

基　　金：中国消化道早癌医师共同成长计划科研项目(GTCZ-2020-AH-34-0008)。

摘　　要：目的:探讨硼酸钠-冰醋酸亚甲蓝染色法在幽门螺旋杆菌(Hp)检测中的价值。方法:选取Hp确诊患者62例,按照Hp感染程度分为轻、中、重度3组分别进行硼酸亚甲蓝染色和硼酸钠-冰醋酸亚甲蓝染色,比较Hp在各组中的检出率。结果:重度组两种染色方法检出率均为100%;中度组硼酸钠-冰醋酸亚甲蓝染色检出率为100%,硼酸亚甲蓝染色检出率为89.47%;轻度组硼酸钠-冰醋酸亚甲蓝染色检出率为90.63%,硼酸亚甲蓝染色检出率为68.75%,且硼酸钠-冰醋酸亚甲蓝检出率高于硼酸亚甲蓝,差异有统计学意义(P<0.05)。结论:轻度感染组中硼酸钠-冰醋酸亚甲蓝染色效果优于硼酸亚甲蓝染色。Objective:To assess the value of sodium borate-methylene blue glacial acetic acid staining method in detecting Helicobacter pylori(Hp)Methods:Sixty-two patients confirmed with Hp infection were included,and divided into three groups by the degree(mild,moderate and severe)of infection.The gastric antrum biopsies were stained by borate methylene blue and sodium borate-methylene blue glacial acetic acid,and compared for the positive rate of the two stains in three groups.Results:100%of Hp infection was detected in the severe group by two stains.The positive rate was 100%and 90.63%,and 89.47%and 68.75%in moderate and mild infection group by staining with sodium borate-methylene blue glacial acetic acid and borate methylene blue,respectively.The detection rate was higher by sodium borate-methylene blue glacial acetic acid than by borate methylene blue(P<0.05).Conclusion:Staining of the biopsy with sodium borate-methylene blue glacial acetic acid is better than that by borate methylene blue in detecting Hp infection.

关 键 词：幽门螺旋杆菌 检出率 硼酸钠-冰醋酸亚甲蓝染色 硼酸亚甲蓝染色 

分 类 号：R573.1[医药卫生—消化系统] R446.6[医药卫生—内科学]