越鞠丸对慢性不可预见性应激导致抑郁样行为模型大鼠学习记忆的影响  

Effects of Yueju pill on the learning and memory depression-like rat models induced by chronic unpredictable stress

作　　者：张芯悦 梁坤 沈国晴 冯清源 李光建 傅丹敏 胡安冉 汪萌芽[2] 黄宏平[2] ZHANG Xinyue;LIANG Kun;SHEN Guoqing;FENG Qingyuan;LI Guangjian;FU Danmin;HU Anran;WANG Mengya;HUANG Hongping(Rising-Star Group,Wannan Medical College,Wuhu 241002,China)

机构地区：[1]皖南医学院启明星小组,安徽芜湖241002 [2]皖南医学院生理学与神经生物学教研室,安徽芜湖241002

出　　处：《皖南医学院学报》2022年第3期289-291,295,共4页Journal of Wannan Medical College

基　　金：国家级大学生创新创业训练计划项目(201810368012)。

摘　　要：目的:观察越鞠丸对慢性不可预见性应激(CUS)导致抑郁样行为模型大鼠空间学习记忆能力的影响。方法:28只雄性SD大鼠(160~190 g)分成正常组(n=9)、抑郁组(n=10)和越鞠丸组(n=9)。其中正常组大鼠不予任何处理,对其余两组进行3周的CUS刺激。通过比较正常组与抑郁组、越鞠丸组旷场和糖水偏爱实验的行为学指标差异检测模型是否制备成功,采用Y迷宫和Morris水迷宫检测大鼠的空间学习记忆能力,对越鞠丸组大鼠给药后,观察行为学指标和迷宫实验的指标差异。结果:①抑郁组与越鞠丸组体质量、糖水偏爱率、旷场实验得分均低于正常组(P<0.05)。在Y迷宫实验中,抑郁组与越鞠丸组自主交替率较正常组降低,新异臂停留时间缩短、新异臂潜伏期延长(P<0.01)。②给药21 d后,越鞠丸组的旷场实验得分较抑郁组提高(P<0.05)。在Y迷宫实验中,越鞠丸组较抑郁组自主交替率、起始臂进入次数和总进臂次数均增加(P<0.05)。③Morris水迷宫实验中,在空间探索阶段,越鞠丸组较抑郁组穿越虚拟平台次数增加(P<0.01),目标象限停留时间延长(P<0.05)。结论:越鞠丸在缓解CUS模型大鼠抑郁样行为的同时,能改善大鼠因应激导致的空间学习记忆障碍。Objective:To observe the effect of Yueju pill on spatial learning and memory in depression-like model rats induced by chronic unpredictable stress.Methods:Twenty-eight male Sprague-Dawley rats(160-190 g)were divided into normal group(n=9),model group(n=10)and Yueju pill treatment group(n=9).Rats in the normal group were free of any intervention,and those in another two groups were induced by chronic unpredictable stress(CUS)to develop the models.Whether the models were successfully prepared was verified using the behavioral indexes,including open field test and sugar water preference experiment,and Y-maze and Morris water maze were used to evaluate the spatial learning and memory in rats in each group.Additionally,rats in Yueju pill treatment group were observed for the difference of behavioral indicators and tested results of Y-maze and Morris water maze before and after administration of Yueju pill.Results:①The body weight,percentage of sucrose preference and open-field test scores were significantly decreased in rats in model group and Yueju pill treatment group(P<0.05),and decreased spontaneous alternation rate,reduced novel arm stay,yet prolonged latency at the new arm were seen in rats in model group and Yueju pill treatment group in Y-maze test compared to the normal group(all P<0.01);②By the 21 st day of medication,rats in the Yueju pill treatment group had higher scoring in open-field test than in those in model group(P<0.05),and Yueju pill treatment resulted in increased spontaneous alternation rate,the number of entry original arm and total number of arms in Y-maze test(P<0.05);③In Morris water maze retention stage,the number of crossing virtual platform as well as performance time in target zone of space exploration test were increased in rats in Yueju pill treatment group(P<0.05).Conclusion:Yueju pill can improve the depression-like behavior and spatial learning and memory in model rats induced by chronic unpredictable stress.

关 键 词：越鞠丸 抑郁症 Y迷宫 MORRIS水迷宫 学习记忆 

分 类 号：R749.4[医药卫生—神经病学与精神病学] R285.5[医药卫生—临床医学]