Effects of phosvitin phosphopeptide-Ca complex prepared by efficient enzymatic hydrolysis on calcium absorption and bone deposition of mice  

作　　者：Mengdie Zhao Dong Uk Ahn Songming Li Wei Liu Shengwei Yi Xi Huang 

机构地区：[1]National Research and Development Center for Egg Processing,College of Food Science and Technology,Huazhong Agricultural University,Wuhan 430070,China [2]Animal Science Department,Iowa State University,Ames 50011,USA [3]Chongqing Institute for Food and Drug Control,Chongqing 401121,China

出　　处：《Food Science and Human Wellness》2022年第6期1631-1640,共10页食品科学与人类健康（英文）

基　　金：supported by the General Program of National Natural Science Foundation of China(32072237);the Hubei Provincial Natural Science Foundation of China(2020CFB583);Fundamental Research Funds for the Central Universities(2662020SPPY006)。

摘　　要：Phosvitin(PV)was treated with high-temperature,mild pressure(HTMP),and enzyme combination,and then phosvitin phosphopeptides-calcium(PPP-Ca)complexes were prepared.The low-calcium specific pathogen free-Kunming(SPF-KM)mice were used to determine the effect of PPP-Ca complexes on intestinal calcium absorption and their utilization for bone formation.The serum calcium content was the highest with the HTMP-Enz-PPP-Ca treatment(2.19 mmol/L),and it significantly down-regulated the abnormal elevation of serum alkaline phosphatase(AKP)caused by calcium deficiency.The low-calcium control group had the lowest calcium deposited to the femur(80.41 mg/g)and the lowest femur bone mineral density(BMD)(0.17 g/cm^(3)),while HTMP-Enz-PPP-Ca significantly improved bone calcium content(94.33 mg/g)and BMD(0.29 g/cm^(3)).The micro-computed tomography(MCT)images showed that the femur with the normal control,PV-Ca,and HTMP-Enz-PPP-Ca treatments had a more compact,complete,and thicker trabecular network than the low-calcium and CaCl_(2)treatments.These results indicated that the organic calcium(HTMP-Enz-PPP-Ca)promoted calcium absorption and bone deposition,and the effect of HTMP-Enz-PPP-Ca was better than the inorganic CaCl_(2).

关 键 词：Phosvitin phosphopeptide(PPP)-Ca complex SPF KM mice Calcium absorption Bone formation 

分 类 号：TS201.4[轻工技术与工程—食品科学]