Identifi cation and characterization of a novel tetrapeptide from enzymatic hydrolysates of Baijiu byproduct  

作　　者：Qiang Wu Changqing Zhong Guirong Zeng Xu Zhang Liping Xiang Chang Wan Yougui Yu 

机构地区：[1]College of Food and Chemical Engineering,Shaoyang University,Shaoyang 422000,China [2]Department of Cardiovascular Medicine,Hunan Provincial People's Hospital,Changsha 410005,China [3]Hunan Experimental Animal Center,Hunan Drug Safety Evaluation and Research Center,Liuyang 410331,China

出　　处：《Food Science and Human Wellness》2022年第6期1641-1649,共9页食品科学与人类健康（英文）

基　　金：sponsored by the Outstanding Youth Project of Hunan Education Department(19B505);Changsha City Science and Technology Plan Project(kq2004113);the 2020 Natural Science Foundation of Hunan Province of China(2020JJ8061);the 2020 Science and Technology Innovation Project of Hunan Province of China(2020SK50921);the Undergraduate Inquiry Learning and Innovative Experimental Project of Hunan Education Department(2019[100]-1886)。

摘　　要：In order to prepare angiotensin I-converting enzyme(ACE)inhibitory peptides,distilled spent grains of Chinese strong-flavor Baijiu were hydrolyzed by alcalase followed by papain under optimized conditions.A superior ACE inhibitory peptide was separated and purifi ed by ultrafi ltration and high-performance liquid chromatography(HPLC),and its amino acid sequence was further identified as Gln-Gly-Val-Pro(QGVP)by electrospray mass spectrometry(ESI-MS).QGVP formed 6 hydrogen bonds with the active site of ACE,which is responsible for reducingα-helix structure content of ACE causing subsequent inactivation.M oreover,it showed no significant cytotoxicity toward human umbilical vein endothelial cells(HUVECs),a nd signifi cantly i nduced phosphorylation of endothelial nitric oxide synthase(p-e NOS)and decreased endothelin 1(END1)expression in angiotensin I(Ang I)-treated HUVECs,demonstrating the potential antihypertensive effect.The peptide QGVP hydrolyzed from distilled spent grain proteins of Chinese strong-fl avor Baijiu was expected to be used as a food ingredient to prevent or co-treat hypertension with other chemical drugs.

关 键 词：Baijiu Distilled spent grain ACE inhibitory peptide Inhibition mechanism 

分 类 号：TS261.9[轻工技术与工程—发酵工程]