细胞蜡块联合CEA、TTF-1、E-cadherin在肺腺癌胸腔积液病理诊断中的临床价值研究  

Research on the clinical value of cell wax block combined with CEA,TTF-1 and E-cadherin in the pathological diagnosis of pleural effusion of lung adenocarcinoma

作　　者：赖丽雅 廖志东[1] 赖骏威 郑渝兰 谢运红 曹浩林 LAI Liya;LIAO Zhidong;LAI Junwei;ZHENG Yulan;XIE Yunhong;CAO Haolin(Department of Pathology,Meizhou Hospital of Traditional Chinese Medicine,Guangdong,Meizhou 514011,China)

机构地区：[1]广东省梅州市中医医院病理科,广东梅州514011

出　　处：《中国医药科学》2022年第12期130-133,共4页China Medicine And Pharmacy

基　　金：广东省梅州市社会发展科技计划项目(201111102050554)。

摘　　要：目的探究细胞蜡块联合癌胚抗原(CEA)、甲状腺转录因子1(TTF-1)、上皮钙黏素(E-cadherin)在肺腺癌胸腔积液病理诊断中的临床价值。方法选择2020年1月至2021年7月在梅州市中医医院进行病理形态学诊断且有病理诊断结果的100例患者的胸腔积液标本作为研究对象。所有胸腔积液均行细胞涂片,剩余胸腔积液沉渣制成细胞蜡块,行CEA、TTF-1、E-cadherin免疫组织化学染色。根据病理结果将研究对象分为3组:恶性组(n=35)、可疑组(n=34)、良性组(n=31)。比较各组TTF-1、CEA、E-cadherin表达阳性率,比较各单一指标、联合指标诊断效能。结果恶性组TTF-1、CEA、E-cadherin表达阳性率较良性组高,差异有统计学意义(P<0.05)。可疑肺腺癌TTF-1、CEA、E-cadherin表达阳性率较可疑癌高,差异有统计学意义(P<0.05)。TTF-1、CEA、E-cadherin联合诊断的诊断效能较单一指标高,差异有统计学意义(P<0.05)。免疫细胞化学与细胞病理形态学联合诊断的阳性率高于单一免疫细胞学、细胞病理形态学检测(P<0.05)。结论细胞蜡块联合CEA、TTF-1、E-cadherin在肺腺癌胸腔积液病理诊断中,具有较高的诊断效能。Objective To investigate the clinical value of cell wax block combined with carcinoembryonic antigen(CEA),thyroid transcription factor 1(TTF-1)and E-cadherin in the pathological diagnosis of pleural effusion of lung adenocarcinoma.Methods The pleural effusion samples of 100 patients with pathomorphological diagnosis and pathological diagnosis in Meizhou Hospital of Traditional Chinese Medicine from January 2020 to July 2021 were selected as the research subjects.Cell smear was performed on all pleural effusion,and the residual pleural effusion sediment was prepared into cell wax blocks for immunohistochemical staining with CEA,TTF-1 and E-cadherin.According to the pathological results,the research subjects were divided into the malignant group(n=35),the suspicious group(n=34),and the benign group(n=31).The positive rates of TTF-1,CEA and E-cadherin expressions in each group were compared,and the diagnostic efficiencies of a single index or a combination of indices were compared.Results The positive rates of TTF-1,CEA and E-cadherin expression in the malignant group were higher than those in the benign group,and the difference was statistically significant(P<0.05).The positive rate of TTF-1,CEA and E-cadherin expression in suspected lung adenocarcinoma were higher than that in suspicious carcinoma,and the differences were statistically significant(P<0.05).The diagnostic efficiency of combined diagnosis of TTF-1,CEA and E-cadherin were higher than that of single index diagnosis,and the differences were statistically significant(P<0.05).The positive rate of combined diagnosis of immunocytochemistry and cytopathology was higher than that of single detection of immunocytochemistry and cytopathology(P<0.05).Conclusion The combination of cell wax block with CEA,TTF-1 and E-cadherin in the pathological diagnosis of lung adenocarcinoma pleural effusion has relatively high diagnostic efficiency.

关 键 词：细胞蜡块 CEA TTF-1 E-CADHERIN 肺腺癌 胸腔积液 

分 类 号：R734.2[医药卫生—肿瘤]