参黄膏贴敷神阙穴治疗乳腺癌模型小鼠化疗相关性肠道功能障碍的实验研究  

The experimental study on the treatment of chemotherapy-induced gastrointestinal toxicity in breast cancer model mice with Shenhuang plaster applied to Shenque

作　　者：沈怡琳 陈贵平 朱晨晨 史亚楠 王蓓 徐小宏 Shen Yilin

机构地区：[1]浙江中医药大学第一临床医学院,310053

出　　处：《浙江临床医学》2022年第5期640-643,共4页Zhejiang Clinical Medical Journal

基　　金：浙江省公益性基础研究项目(LCD19C04007)。

摘　　要：目的 探讨参黄膏贴敷乳腺癌模型小鼠神阙穴对紫杉醇化疗所致的胃肠道功能紊乱(CIGT)的影响及可能机制.方法 4T_(1)-Luc乳腺癌细胞株接种至每只小鼠胸部皮下100μL,第10天将32只造模成功的小鼠随机分为对照组、参黄膏组、紫杉醇组和参黄膏+紫杉醇组,每组各8只.紫杉醇组和参黄膏+紫杉醇组隔日腹腔注射0.01 mg/g紫杉醇针,参黄膏组和参黄膏+紫杉醇组取参黄膏贴敷小鼠神阙穴,更换2次/d.干预3周后观察各组小鼠瘤体生长情况、胃肠道传输功能几何均数(GC)、小鼠回肠和结肠HE染色病理学检查和小肠组织TLR4、MyD88和NF-κB mRNA表达水平.结果 与对照组比较,紫杉醇组小鼠体质量下降,胃肠道GC下降;回肠和结肠绒毛高度和黏膜层厚度均降低;小肠IL-6、MyD88和NF-kBmRNA表达水平均增加(P<0.05).与紫杉醇组比较参黄膏+紫杉醇组小鼠体质量增加,胃肠道GC增加;回肠和结肠绒毛长度和黏膜层厚度均增加;小肠IL-6、MyD88和NF-kBmRNA表达水平下降(P<0.05).结论 参黄膏贴敷神阙穴可以改善乳腺癌模型小鼠化疗相关性肠道功能紊乱,其作用机制可能是通过抑制TLR4/MyD88/NF-κB信号通路而发挥作用.Objective To investigate the efficacy of Shenhuang Plaster(SHP)application on Shenque oil chemotherapy-induced gastrointestinal toxicity(CIGT)and its possible mechanism in tumour-bearing mice models.Methods 100 ul breast cancer cell line 4T_(1)-Luc were inoculated to the chest subcutaneous fat pad of mice.Thirty-two successfully-modeled mouse were randomly divided into control group,SHP group,Paclitaxel(PTX)group and SHP+PTX group with 8 mice in each group on the 10th day after inoculation.PTX group and SHP+PTX group were intraperitoneally injected with 0.01 mg/g paclitaxel every 2 days.SHP group and SHP+PTX group were administered with SHP on the animal Shenque twice a day.After 3 weeks treatment,tumor growth,geometric mean of gastrointestinal transit Rinction,pathological examination of mouse ileum and colon with HE staining,and expression levels of TLR4,MyD88 and NF-k B mRNA in small intestine of mice in each group were observed.Results Body weight gain,geometric mean of gastrointestinal transit fiinction,and villi height and mucosa thickness of ileum and colon decreased in PTX group,and the mRNA expression level of IL-6,Myd88 and NF-kB in small intestine increased significantly compared with those of the control group(Pv0.05).The body weight gain,geometric mean of gastrointestinal transit Rinction,and the villi height and mucosal thickness of ileum and colon of the SHP+PTX group were higher than those of PTX group(P<0.05).Meanwhile,the mRNA levels of IL~6,Myd88 and NF-kB in small intestine decreased significantly(P<0.05).Conclusion SHP appbeation on Shenque can improve paclitaxel chemotherapy-induced gastrointestinal toxicity in breast cancer mouse model through inhibiting TLR4/MyD88/NF-KB pathway.

关 键 词：参黄膏 化疗相关性肠道功能障碍 TLR4/MyD88/NF-κB通路 作用机制 

分 类 号：R73[医药卫生—肿瘤]