METTL14在头颈鳞癌发生发展中的作用  

Role of METTL14 in the development and progression of Head and neck squamous cell carcinomas

作　　者：李丹[1] 董凯峰[1] 苏静[1] 薛海涛[1] 张继华[1] 李天客[2] LI Dan;DONG Kaifeng;SU Jing;XUE Haitao;ZHANG Jihua;LI Tianke(Department of Otolaryngology,the First Hospital of Hebei Medical University,Shijiazhuang 050030)

机构地区：[1]河北医科大学第一医院耳鼻咽喉科,石家庄050030 [2]河北医科大学第四医院口腔科

出　　处：《现代口腔医学杂志》2022年第2期78-82,130,共6页Journal of Modern Stomatology

基　　金：河北省卫生和计划生育委员会科研基金资助项目(20201146)。

摘　　要：目的本研究通过检测METTL14在头颈鳞癌中的表达水平,探究其在头颈鳞癌发生发展中的作用,从而揭示METTL14在头颈鳞癌侵袭、转移中发挥的作用。方法于2015年8月至2020年8月期间,收集在河北医科大学第一医院和河北医科大学第四医院就诊的79例头颈鳞癌患者。利用qPCR检测组织中METTL14的mRNA的表达。分别分析临床参数与METTL14基因表达水平之间的相关性,通过计算风险比(HRs)和95%置信区间(95%CI)分析相关参数与METTL14的联系。结果在受试个体中,淋巴转移(P=0.016)和生存时间(P<0.001)与METTL14表达显著相关。斯皮尔曼相关系数显示淋巴转移(ρ=-0.271,P=0.016)、生存时间(ρ=-0.747,P<0.001)与METTL14显著相关。单因素cox回归描述了研究对象在单变量水平上的HRs和95%CI,并得出METTL14表达水平(HR=27.972,95%CI:9.609-81.430,P<0.001)与患者的生存时间有明显相关性。排除混杂因素的影响,多因素回归分析指出METTL14表达水平(HR=30.508,95%CI:10.059-92.531,P<0.001)依然是头颈鳞癌生存的显著危险风险。结论本研究通过检测METTL14在头颈鳞癌中的表达水平,并进一步证实METTL14在头颈鳞癌发生发展中发挥了促进头颈鳞癌细胞增殖的作用,且可能作为预后指标,为头颈鳞癌的早期诊断及治疗提供新靶点。Objective In this study,the expression level of METTL14 in head and neck squamous cell carcinomas(HNSCC)was detected to explore its role in the occurrence and development of HNSCC,so as to reveal the role of METTL14 in the invasion and metastasis of HNSCC.Methods A total of 79 patients with HNSCC were recruited at the Fourth Hospital of Hebei Medical University and First Hospital of Hebei Medical University between August 2015 and August 2020.The expression of METTL14 mRNA in tissues was detected by qPCR.The correlation between clinical parameters and METTL14 gene expression level was analyzed,and the correlation was analyzed by calculating risk ratio(HRs)and 95%confidence interval(95%CI).Results In subjects,lymphoid metastasis(P=0.016)and survival time(P<0.001)were significantly correlated with METTL14 expression.The Spearman correlation coefficient showed a significant correlation between lymphoid metastasis(ρ=-0.271,P=0.016)and survival time(ρ=-0.747,P<0.001)and METTL14.Univariate cox regression described HRs and 95%CI of study subjects at univariate level,and concluded that METTL14 expression level(HR=27.972,95%CI:9.609-81.430,P<0.001)was significantly correlated with patient survival time.After excluding the influence of confusors,the multivariate regression analysis indicated that the expression level of METTL14(HR=30.508,95%CI:10.059-92.531,P<0.001)was still a significant risk factor for HNSCC survival.Conclusion By detecting the expression level of METTL14 in HNSCC,this study further confirmed that METTL14 plays a role in promoting the proliferation of HNSCC cells in the occurrence and development of HNSCC,and may be used as a prognostic indicator,providing a new target for the early diagnosis and treatment of HNSCC.

关 键 词：头颈鳞癌 METTL14 预后 生存 基因治疗 

分 类 号：R739.8[医药卫生—肿瘤]