补肾活血方对激素性股骨头缺血性坏死小鼠肾素-血管紧张素系统经典轴的影响  

Efficacy of Bushen Huoxue Recipe on ACE/AngⅡ/AT1R of RAS Classical Axis in Steroid Induced Ischemic Necrosis of Femoral Head in Mice

作　　者：帅波 马陈 沈霖[1] 杨艳萍[1] 卢俊杰 杨功旭[2] 孙敏[2] SHUAI Bo;MA Chen;SHEN Lin;YANG Yanping;LU Junjie;YANG Gongxu;SUN Min(Department of Integrated Traditional Chinese Medicine and Western Medicine,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,China;Department of Orthopaedics,Hubei Provincial Hospital of Traditional Chinese Medicine,Wuhan 430074,China)

机构地区：[1]华中科技大学同济医学院附属协和医院中西医结合科,武汉430022 [2]湖北省中医院骨科

出　　处：《中国中医骨伤科杂志》2022年第6期1-5,共5页Chinese Journal of Traditional Medical Traumatology & Orthopedics

基　　金：国家自然科学基金(81974546,82174182,82004201);中央高校基本科研业务费专项资金资助项目(2019kfyRCPY093)。

摘　　要：目的:初步探讨补肾活血方对激素性股骨头缺血性坏死(INFH)小鼠肾素-血管紧张素系统(RAS)经典轴血管紧张素转换酶(ACE)/血管紧张素Ⅱ(AngⅡ)/血管紧张素Ⅱ-1型受体(AT1R)的影响。方法:将60只SPF级C57BL/6雄性小鼠随机分5组,即健康对照组、激素性INFH模型组、补肾活血方组、骨化三醇组及阿利吉仑组。治疗3个月后,ELISA法检测循环血中1,25(OH)_(2)D_(3)及AngⅡ的含量,并检测股骨头局部VDR、ACE、AT1R mRNA表达情况,行免疫组化分析。结果:与模型组相比,健康对照组、补肾活血方组、骨化三醇组及阿利吉仑组循环血中1,25(OH)_(2)D_(3)含量明显升高,差异有统计学意义(P<0.05),而AngⅡ含量明显降低,差异有统计学意义(P<0.05)。与模型组相比,健康对照组、补肾活血方组、骨化三醇干预组及阿利吉仑组骨组织局部VDR mRNA的表达明显升高,差异有统计学意义(P<0.05),而ACE、AT1R表达明显降低,差异有统计学意义(P<0.05)。免疫组化结果显示:模型组骨组织中ACE及AT1R阳性表达较其他各组高,差异有统计学意义(P<0.05),而VDR阳性表达较其他各组低,差异有统计学意义(P<0.05)。结论:激素性INFH小鼠RAS经典轴ACE/AngⅡ/AT1R被激活,补肾活血方可激活1,25(OH)_(2)D_(3)/VDR信号通路,并能抑制激活的RAS经典轴ACE/AngⅡ/AT1R,这可能是补肾活血方防治激素性INFH的部分机制。Objective:To study the efficacy of Bushen Huoxue recipe on ACE/AngⅡ/AT1R of steroid induced ischemic necrosis of femoral head(INFH)in mice.Methods:All 60 SPF C57BL/6 male mice were randomly divided into 5 groups:control group,steroid induced INFH model group,Bushen Huoxue recipe group,calcitriol intervention group and aliskiren intervention group.The levels of 1,25(OH)_(2)D_(3) and AngⅡin the circulating blood were detected by ELISA method;the expression of VDR,ACE,AT1R mRNA in the femoral head was detected,and immunohistochemistry was performed three months after treatment.Results:Compared with the model group,the content of 1,25(OH)_(2)D_(3) in the circulating blood of the healthy control group,Bushen Huoxue recipe group,calcitriol intervention group and aliskiren group was increased significantly(P<0.05),while the content of AngⅡwas decreased significantly(P<0.05).Compared with the model group,the expression of VDR mRNA in bone tissue of healthy control group,Bushen Huoxue recipe group,calcitriol intervention group and aliskiren group was increased significantly(P<0.05),while the expression of ACE and AT1R was decreased significantly(P<0.05).The immunohistochemistry results were showed that the expression of ACE and AT1R in bone tissue of model group was higher than that of other groups(P<0.05),while the expression of VDR was lower than that of other groups(P<0.05).Conclusion:ACE/AngⅡ/AT1R of RAS classic axis in steroid induced INFH mice is activated.Bushen Huoxue recipe can activate 1,25(OH)_(2)D_(3)/VDR signal pathway and inhibit the activated ACE/AngⅡ/AT1R of RAS classic axis,which may be part of the mechanism of Bushen Huoxue recipe in the prevention and treatment of steroid induced INFH.

关 键 词：激素性股骨头坏死 补肾活血方 维生素D受体mRNA 血管紧张素 血管紧张素转换酶 

分 类 号：R-33[医药卫生]