不同贮藏条件对芹菜叶绿素含量的影响及相关基因表达分析  

Effects of Different Storage Conditions on the Content of Chlorophyll and Expression Analysis of Related Genes in Celery(Apium graveolens)

作　　者：贾敏 朱胜琪 王雅慧 谭杉杉 刘洁霞 熊爱生[1] JIA Min;ZHU Sheng-Qi;WANG Ya-Hui;TAN Shan-Shan;LIU Jie-Xia;XIONG Ai-Sheng(College of Horticulture,Nanjing Agricultural University,State Key Laboratory of Crop Genetics and Germplasm Enhancement,Key Laboratory of Biology and Germplasm Enhancement of Horticultural Crops in East China,Ministry of Agriculture and Rural Affairs,Nanjing 210095,China)

机构地区：[1]南京农业大学园艺学院/作物遗传与种质创新国家重点实验室/农业农村部华东地区园艺作物生物学与种质创制重点实验室,南京210095

出　　处：《农业生物技术学报》2022年第6期1078-1086,共9页Journal of Agricultural Biotechnology

基　　金：国家自然科学基金(31272175);江苏省农业自主创新资金(CX(18)2007);江苏高校优势学科建设项目(PAPD)。

摘　　要：芹菜(Apium graveolens)是重要的叶菜类蔬菜,具有很高的营养价值和多种药用功能。采后芹菜贮藏过程中的叶绿素含量是评价芹菜品质的重要指标之一。采后不同贮藏条件对芹菜叶绿素含量的影响及其分子机制尚不清楚。本研究以’四季小香芹’和’六合黄心芹’为实验材料,设置室温(25℃)、低温(4℃)和室温黑暗(25℃)3个不同的贮藏条件,分别测定0、6、24、30、48和54 h的芹菜叶片叶绿素含量,并通过荧光定量PCR检测叶绿素代谢相关基因(AgMPE,AgPPH,AgPAO,AgCAO,AgNOL和AgHCAR)的表达水平。结果显示,低温条件下叶绿素a和叶绿素b含量的均值最高;除24和30 h以外,叶绿素总积累量均在低温贮藏时最高;室温黑暗条件下总叶绿素含量高于室温条件;AgCAO基因的转录水平随贮藏时间的增加发生显著的变化,且与叶绿素b的积累模式一致。研究结果表明,低温贮藏条件抑制了芹菜叶绿素的降解,能够更好地保持芹菜叶绿素含量,维持芹菜的营养品质和感官品质。本研究为探究采后芹菜叶绿素的积累规律及相关基因的调控模式提供了参考。Celery(Apium graveolens)is an important leafy vegetable with high nutritional values and numerous medicinal functions.Chlorophyll content is one of the important indexes to evaluate the quality of celery during storage after harvest.The influence on the content of chlorophyll in celery under different postharvest environmental conditions is still unclear.In this study,’Sijixiaoxiangqin’and’Liuhehuangxinqin’were stored at 3 different storage conditions of room temperature(25℃),low temperature(4℃)and darkness at room temperature(25℃).The chlorophyll contents and the expression levels of metabolism-related genes(AgMPE,AgPPH,AgPAO,AgCAO,AgNOL and AgHCAR)were detected at 0,6,24,30,48 and 54 h after treatment.The results showed that the average contents of chlorophyll a and chlorophyll b were the highest under low temperature treatment,and total accumulation of chlorophyll was the highest at low temperature storage except for 24 and 30 h,and the peak of total chlorophyll content appeared at low temperature.The total chlorophyll content under darkness at room temperature condition was higher than that under room temperature condition.The transcription level of AgCAO gene changed significantly with the increase of storage time,which was consistent with the accumulation pattern of chlorophyll b.The results indicated that low temperature condition inhibited the degradation of chlorophyll content and could better maintain the chlorophyll content of celery.Low temperature could maintain the nutritional and sensory quality of celery.This study provides a reference for exploring the regulation pattern of chlorophyll accumulation and related genes in celery after harvest.

关 键 词：芹菜 低温 叶绿素 基因表达 贮藏 

分 类 号：S636.3[农业科学—蔬菜学]