年龄对小鼠植入前胚胎Dnmts表达量的影响  

Effects of aging on the expression of Dnmts in developmental stages of mouse preimplantation embryos

作　　者：岳明星 简伟杰 李浡[1] 陈思[1] 危晴[1] 陈亮[1] 辛秀兰[1] YUE Mingxing;JIAN Weijie;LI Bo;CHEN Si;WEI Qing;CHEN Liang;XIN Xiulan(Beijing Electronic Technology Vocational College,Beijing 100176,China)

机构地区：[1]北京电子科技职业学院,北京100176

出　　处：《黑龙江动物繁殖》2022年第3期51-55,共5页Heilongjiang journal of animal reproduction

基　　金：2017年北京电子科技职业学院校级重点课题“老化对小鼠卵母细胞和早期胚胎中DNA甲基转移酶表达量化和组蛋白甲基化状态的影响”(2017Z005-012-KXB);2018年北京电子科技职业学院校级重点课题“玻璃化冷冻对小鼠卵母细胞和早期胚胎及其后代DNA甲基化的影响”(2018Z002-011-KXB);企业横向项目“动物胚胎冷冻保存”(2021H097-KQ);天然产物国家标准样品研制及产品开发技术研究项目(SG030801)。

摘　　要：为了研究老化对小鼠植入前不同时期胚胎DNA甲基转移酶(Dnmts)表达量的影响,试验利用Western-blot方法分别检测了青、老年小鼠2细胞/4细胞、8细胞/桑椹胚、囊胚三种胚胎的Dnmts(Dnmt3a、Dnmt1、Dnmt3b、Dnmt3L)表达量。结果表明:青年和老年小鼠2细胞/4细胞胚胎除Dnmt3a表达量差异不显著(P>0.05)外,其余3种DNA甲基转移酶(Dnmt1、Dnmt3b、Dnmt3L)表达量均随年龄增加呈显著下降趋势(P<0.05);在8细胞/桑椹胚和囊胚中青年和老年小鼠4种DNA甲基转移酶表达量均无显著差异(P>0.05)。说明老化会导致小鼠2细胞和4细胞胚胎Dnmts表达量产生波动,对其他时期胚胎影响不大。To study the effect of aging on the expression of Dnmts in developmental stages of mouse preimplantation embryos, Western-blot was used to detect the expression of Dnmts in 2-cell, 4-cell, 8-cell embryos, morula and blastocysts of young and old mice, respectively. The results showed that no significant difference was found in Dnmt3 a expression between 2-cell and 4-cell embryos(P>0.05), however, the expression levels of Dnmt1, Dnmt3 b and Dnmt3 were found with a significant decrease in old mice(P<0.05). No significant difference was found in the expression levels of four DNA methyltransferases in 8-cell embryos, morula and blastocyst(P>0.05). It could be deduced that the fluctuation of Dnmts expression could be affected by aging in 2-cell, 4-cell embryos of mice.

关 键 词：胚胎 DNA甲基转移酶 WESTERN-BLOT 老化 小鼠 

分 类 号：Q786[生物学—分子生物学]