The transcription factors TLR1 and TLR2 negatively regulate trichome density and artemisinin levels in Artemisia annua  

作　　者：Zongyou Lv JinXing Li Shi Qiu Fei Qi Hang Su Qitao Bu Rui Jiang Kexuan Tang Lei Zhang Wansheng Chen 

机构地区：[1]Research and Development Center of Chinese Medicine Resources and Biotechnology,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China [2]Department of Pharmacy,Changzheng Hospital,Second Military Medical University,Shanghai 200003,China [3]Department of Pharmaceutical Botany,School of Pharmacy,Second Military Medical University,Shanghai 200433,China [4]Joint International Research Laboratory of Metabolic&Developmental Sciences,Key Laboratory of Urban Agriculture(South)Ministry of Agriculture,Plant Biotechnology Research Center,Fudan‐SJTU‐Nottingham Plant Biotechnology R&D Center,Shanghai Jiao Tong University,Shanghai 200240,China [5]State Key Laboratory of Subtropical Silviculture,Zhejiang A&F University,Hangzhou 311300,China

出　　处：《Journal of Integrative Plant Biology》2022年第6期1212-1228,共17页植物学报（英文版）

基　　金：funded by the National Key R&D Program of China (2019YFC1711100);National Natural Science Foundation of China (32070332);Shanghai Natural Science Foundation in China (20ZR1453800);Shanghai local Science and Technology Development Fund Program guided by the Central Government (YDZX20203100002948)

摘　　要：The important antimalarial drug artemisinin is biosynthesized and stored in Artemisia annua glandular trichomes and the artemisinin content correlates with trichome density;however,the factors affecting trichome development are largely unknown.Here,we demonstrate that the A.annua R2R3 MYB transcription factor TrichomeLess Regulator 1(TLR1)negatively regulates trichome development.In A.annua,TLR1 overexpression lines had 44.7%–64.0%lower trichome density and 11.5%–49.4%lower artemisinin contents and TLR1-RNAi lines had 33%–93.3%higher trichome density and 32.2%–84.0%higher artemisinin contents compared with non-transgenic controls.TLR1 also negatively regulates the expression of anthocyanin biosynthetic pathway genes in A.annua.When heterologously expressed in Arabidopsis thaliana,TLR1 interacts with GLABROUS3a,positive regulator of trichome development,and represses trichome development.Yeast two-hybrid and pull-down assays indicated that TLR1 interacts with the WUSCHEL homeobox(WOX)protein AaWOX1,which interacts with the LEAFY-like transcription factor TLR2.TLR2 overexpression in Arabidopsis and A.annua showed that TLR2 reduces trichome development by reducing gibberellin levels.Furthermore,artemisinin contents were 19%–43%lower in TLR2-overexpressing A.annua plants compared to controls.These data indicate that TLR1 and TLR2 negatively regulate trichome density by lowering gibberellin levels and may enable approaches to enhance artemisinin yields.

关 键 词：ARTEMISININ Artemisia annua L. LFY MYB transcription factor TRICHOME WOX 

分 类 号：S567.219[农业科学—中草药栽培]