The bacterial effector AvrRxo1 inhibits vitamin B6 biosynthesis to promote infection in rice  

作　　者：Haifeng Liu Chongchong Lu Yang Li Tao Wu Baogang Zhang Baoyou Liu Wenjie Feng Qian Xu Hansong Dong Shengyang He Zhaohui Chu Xinhua Ding 

机构地区：[1]State Key Laboratory of Crop Biology,Shandong Provincial Key Laboratory for Biology of Vegetable Diseases and Insect Pests,College of Plant Protection,Shandong Agricultural University,Tai an,271018 Shandong,PR China [2]College of Agronomy,Shandong Agricultural University,Tai an,271018 Shandong,PR China [3]Department of Energy Plant Research Laboratory,Michigan State University,East Lansing,MI 48824,USA [4]Department of Biology,Duke University,Durham,NC 27708,USA [5]Howard Hughes Medical Institute,Duke University,Durham,NC 27708,USA [6]State Key Laboratory of Hybrid Rice,College of Life Sciences,Wuhan University,Wuhan,430072 Hubei,PR China

出　　处：《Plant Communications》2022年第3期89-101,共13页植物通讯（英文）

基　　金：This study was supported by the National Natural Science Foundation(31872925 and 32072500);Natural Science Outstanding Youth Fund of Shandong Province(JQ201807);Shandong Province Key Research and Development Plan(2019JZZY020608,2020CXGC010803,and 2019GN C106152);Science and Technology Support Plan for Youth Innovation of Colleges and Universities of Shandong Province(2019KJF023);the National Key Research and Development Program of China(2016YFD0100903);X.D.thanks S.H.for hosting his research visit at Mich-igan State University,supported by the United States National Institute of General Medical Sciences(GM109928).

摘　　要：Xanthomonas oryzae pv.oryzicola(Xoc),which causes rice bacterial leaf streak,invades leaves mainly through stomata,which are often closed as a plant immune response against pathogen invasion.How Xoc overcomes stomatal immunity is unclear.Here,we show that the effector protein AvrRxo1,an ATPdependent protease,enhances Xoc virulence and inhibits stomatal immunity by targeting and degrading rice OsPDX1(pyridoxal phosphate synthase),thereby reducing vitamin B6(VB6)levels in rice.VB6 is required for the activity of aldehyde oxidase,which catalyzes the last step of abscisic acid(ABA)biosynthesis,and ABA positively regulates rice stomatal immunity against Xoc.Thus,we provide evidence supporting a model in which a major bacterial pathogen inhibits plant stomatal immunity by directly targeting VB6 biosynthesis and consequently inhibiting the biosynthesis of ABA in guard cells to open stomata.Moreover,AvrRxo1-mediated VB6 targeting also explains the poor nutritional quality,including low VB6 levels,of Xoc-infected rice grains.

关 键 词：rice bacterial leaf streak EFFECTOR stomatal immunity pyridoxal phosphate synthase vitamin B6(VB6) abscisic acid(ABA) 

分 类 号：S511[农业科学—作物学]