甲状腺乳头状癌钠碘转运体表达与BRAF^(V600E)突变的相关性研究  

Correlation between sodium iodine transporter expression and BRAF^(V600E) mutation in thyroid papillary carcinoma

作　　者：李小妹 王利利 李想 杜丽艳 LI Xiao-mei;WANG Li-li;LI Xiang;DU Li-yan(Department of Head and Neck Mammary Gland,The First Affiliated Hospital of Xingtai Medical College,Xingtai Hebei 054001,China;B Ultrasound Room,The First Affiliated Hospital of Xingtai Medical College,Xingtai Hebei 054001,China;Department of Surgical Anaesthesia,The First Affiliated Hospital of Xingtai Medical College,Xingtai Hebei 054001,China)

机构地区：[1]邢台医学高等专科学校第一附属医院头颈乳腺科,河北邢台054001 [2]邢台医学高等专科学校第一附属医院B超室,河北邢台054001 [3]邢台医学高等专科学校第一附属医院手术麻醉科,河北邢台054001

出　　处：《蚌埠医学院学报》2022年第6期783-786,共4页Journal of Bengbu Medical College

基　　金：河北省邢台市科技局科技计划项目(2019ZC326)。

摘　　要：目的:探究甲状腺乳头状癌(PTC)中常见的遗传学事件BRAF^(V600E)突变与钠碘转运体(NIS)表达的关系。方法:选取104例行手术治疗的PTC病人为观察对象,留取术中新鲜PTC组织和癌旁正常组织标本,通过荧光定量PCR法检测NISmRNA含量,石蜡包块行免疫组织化学检测NIS蛋白表达,同时行BRAF^(V600E)突变基因检测。结果:104例PTC组织中BRAF^(V600E)基因突变型共70例(67.3%),高于癌旁正常组织0例(P<0.01);PTC组织中NISmRNA的表达浓度低于癌旁正常组织(P<0.01),但NIS蛋白表达强度则高于癌旁正常组织(P<0.01);PTC组织中NISmRNA表达下调同时NIS蛋白表达上调的比例高于癌旁正常组织(P<0.01);PTC BRAF^(V600E)基因突变病人NISmRNA表达浓度低于野生型病人(P<0.01),但NIS蛋白比较差异无统计学意义(P>0.05)。结论:BRAF^(V600E)基因突变仅存在于癌组织中,可能与癌组织中NISmRNA低表达及NIS蛋白高表达具有相关性,且推测PTC中NIS蛋白可能是主要表达于细胞质中,影响其正常运输功能而导致病人无法聚碘。Objective:To explore the relationship between BRAF^(V600E) mutation and sodium iodide transporter(NIS)in common genetic events in thyroid papillary carcinoma(PTC).Methods:A total of 104 PTC patients who underwent surgical treatment PTC were selected.Fresh PTC tissue and normal tissue adjacent to carcinoma specimens were collected.The contents of NIS mRNA were detected by fluorescence quantitative PCR.NIS protein expressions in paraffin blocks were detected by immunohistochemistry,at the same time BRAF^(V600E) gene mutation was tested.Results:In 104 cases of PTC,there were 70 cases of BRAF^(V600E) gene mutation(67.3%),which was significantly higher than that in the normal tissue adjacent to carcinoma(0 case)(P<0.01).The expression of NIS mRNA in PTC tissues was significantly lower than that in the normal tissues adjacent to carcimoma(P<0.01),but the expression of NIS protein was significantly higher than that in the normal tissues adjacent to carcimoma(P<0.01).The proportion of NIS mRNA expression in the PTC tissues was lower and the increase of NIS protein expression was significantly higher than that in the normal tissues adjacent to carcimoma(P<0.01).The expression of NIS mRNA in patients with gene mutation of PTC BRAF^(V600E) was significantly lower than that in the wild type(P<0.01),but there was no significant difference in NIS protein(P>0.05).Conclusions:BRAF^(V600E) mutation only exists in the cancer tissue,which may be associated with the low expression of NIS mRNA in cancer tissue and high expression of the NIS protein.NIS protein in PTC may be mainly expressed in the cytoplasm,which affects the normal function of transportation and causes patients to be unable to gather iodine.

关 键 词：甲状腺乳头状癌 钠碘转运体 BRAF^(V600E)基因 

分 类 号：R736.1[医药卫生—肿瘤]