抗坏血酸对铜胁迫蚕豆种子萌发和根尖细胞损伤的缓解作用  

Remission Effect of Ascorbic Acid on Seed Germination and Root Tip Cell Damage of Vicia faba under Copper Stress

作　　者：秦永燕 王雪婷 班子茹 宋晓丹 QIN Yongyan;WANG Xueting;BAN Ziru;SONG Xiaodan(Department of Life Sciences,Changzhi College,Changzhi,Shanxi 046001,China)

机构地区：[1]长治学院生命科学系,山西长治046011

出　　处：《农业研究与应用》2022年第2期12-17,共6页Agricultural Research and Application

基　　金：国家自然科学基金项目(41601027);长治学院校级科研课题(XJ2020002302);山西省1331重点学科建设计划经费资助(1331KSC)。

摘　　要：以蚕豆为试验材料,比较不同浓度的铜单独处理(T1)和铜与20 mg/L抗坏血酸(Ascorbic Acid,AsA)联合处理(T2)后蚕豆种子的萌发,胚根长、胚芽长、根系活力、以及蚕豆根尖细胞有丝分裂指数、染色体畸变率和微核千分率,研究抗坏血酸对铜胁迫蚕豆种子萌发和根尖细胞损伤的缓解作用。结果表明:(1)T1和T2组中,随着铜离子浓度的增加,铜对蚕豆种子萌发和早期生长以及蚕豆根尖细胞学观察均表现出一定的抑制作用;(2)T2与T1组相比,蚕豆种子萌发,胚根长、胚芽长、根系活力、以及蚕豆根尖有丝分裂指数均有不同程度的增加,畸变率和微核率有不同程度的下降。因此,在实验浓度范围内,抗坏血酸可以缓解铜胁迫蚕豆种子萌发和根尖细胞损伤的作用。Vicia faba seeds were treated with different concentrations of copper alone(T1)and with copper combining 20 mg/l ascorbic acid(T2).The seed germination,radicle length,germ length,root vitality,root tip cell mitotic index,chromosome aberration rate and micronucleus rate of Vicia faba were compared to study the remission effect of ascorbic acid on seed germination and root tip cell damage under copper stress.The results showed that:1.In T1 and T2 groups,with the increase of copper ion concentration,copper showed certain inhibitory effect on the seed germination,early growth,and root tip cells of Vicia faba.2.Compared with that in T1 group,the seed germination,radicle length,germ length,root vitality,and root tip cell mitotic index in T2,have increased to varying degrees,and the distortion rate and micronucleus rate have decreased to some extent.Therefore,in the experimental concentration range,ascorbic acid can alleviate the effect of copper stress on seed germination and root tip cell damage of Vicia faba.

关 键 词：抗坏血酸 铜胁迫 蚕豆 种子萌发 有丝分裂指数 微核率 染色体畸变 

分 类 号：S643.6[农业科学—蔬菜学] X524[农业科学—园艺学]