PUMA基因在急性髓系白血病患者表达水平及与预后的相关分析  

The expression level of PUMA gene in patients with acute myeloid leukemia and Its correlation with prognosis

作　　者：林樟萍 王可环[2] 赵莲 王小花 谭三阳 LIN Zhangping;WANG Kehuan;ZHAO Lian;WANG Xiaohua;TAN Sanyang(Department of Laboratory Medicine,Hainan Women and Children Medical Center,Haikou,Hainan,China,570206;Department of Laboratory Medicine,Hainan General Hospital,Hainan Hospital Affiliated to Hainan Medical College,Haikou,Hainan,China,570311;Department of Laboratory Medicine,the First Affiliated Hospital of Hainan Medical College,Haikou,Hainan,China,570102;Department of Gynecology,Hainan Women and Children Medical Center,Haikou,Hainan,China,570706;Department of Laboratory Medicine,Haikou Maternal and Child Health Hospital,Haikou,Hainan,China,570203)

机构地区：[1]海南省妇女儿童医学中心检验科,海南海口570206 [2]海南省人民医院海/南医学院附属海南医院检验科,海南海口570311 [3]海南医学院第一附属医院检验科,海南海口570102 [4]海南省妇幼儿童医学中心妇科,海南海口570706 [5]海南省海口市妇幼保健院检验科,海南海口570203

出　　处：《分子诊断与治疗杂志》2022年第6期961-965,共5页Journal of Molecular Diagnostics and Therapy

基　　金：海南省临床医学中心建设项目资助(琼卫医函【2021】75号)。

摘　　要：目的 评估急性髓系白血病(AML)患者PUMA基因表达水平,探讨PUMA基因与AML患者预后的相关性。方法 选取2015年1月至2016年6月本院收治的100例AML患者为研究对象,同时选取急性淋巴细胞白血病(ALL)患者骨髓标本100例为对照组。采用实时荧光定量PCR检测研究对象骨髓中PUMA基因表达水平。分析PUMA基因表达水平与临床疗效的相关性。Kaplan-Meier生存分析PUMA基因与AML预后的相关性。结果 AML组PUMA基因阳性率及mRNA表达量低于对照组,差异具有统计学意义(P<0.05)。PUMA基因各分型阳性表达率为M2>M1>M5>M4>M6,差异具有统计学意义(P<0.05)。PUMA基因各分型mRNA表达量为M2>M1>M5>M4>M6,差异具有统计学意义(P<0.05)。PUMA基因高表达AML患者的临床疗效显著优于PUMA基因低表达AML患者,差异具有统计学意义(P<0.05)。随访3年,M1、M2、M4、M5及M6各类型AML患者生存率比较差异无统计学意义(P>0.05),但同一类型AML患者PUMA基因高表达的生存率高于PUMA基因低表达患者,差异具有统计学意义(P<0.05)。PUMA高表达的AML患者OS优于PUMA低表达的AML患者,差异具有统计学意义(P<0.05)。PUMA基因高表达患者的化疗后感染、复发率低于PUMA基因低表达患者,差异具有统计学意义(P<0.05),但髓外浸润发生率比较差异无统计学意义(P>0.05)。结论 AML患者骨髓中PUMA基因低表达,与患者化疗疗效和预后存在相关性。Objective To evaluate the expression level of p53 up-regulated modulator of apoptosis(PUMA)gene in patients with acute myeloid leukemia(AML),and to analyze the relationship between PUMA gene and prognosis of patients with AML. Methods 100 patients with AML in our hospital from June 2015 to January 2016 were selected. 100 cases of bone marrow specimens from patients with acute lymphoblastic leukemia(ALL)were selected to the control group. The expression of PUMA gene in bone marrow was detected by real-time fluorescence quantitative PCR. The correlation between PUMA gene andclinical efficacy was analyzed. The correlation between PUMA gene and prognosis of AML was analyzed by Kaplan-Meier survival analysis. Results Compared to the control group,the positive rate of PUMA gene and the expression levels of PUMA m RNA in the AML group were lower(P<0.05). The positive expression rates of PUMA genotypes were:M2>M1>M5>M4>M6(P<0.05). The m RNA of each type of PUMA was:M2>M1>M5>M4>M6(P<0.05). The clinical efficiency of high expression levels of PUMA gene was better than that of AML patients with low PUMA gene expression(P<0.05). After 3 years of follow-up,there was no significant difference in the survival rate of M1,M2,M4,M5 and M6 of AML patients(P>0.05),but the survival rate of patients with high PUMA gene expression in the same type of AML was higher than that of patients with low PUMA gene expression,the difference was statistically significant(P<0.05). The OS of AML patients with high PUMA expression was better than that of AML patients with low PUMA expression,and the difference was statistically significant(P<0.05). The infection and recurrence rates after chemotherapy in patients with high PUMA gene expression were lower than those in patients with low PUMA gene expression,and the difference was statistically significant(P<0.05),but there was no significant difference in the incidence of extramedullary infiltration(P>0.05). Conclusions The low expression level of PUMA gene in the bone marrow of AML is is associated

关 键 词：急性髓系白血病 PUMA基因 Kaplan-Meier曲线 

分 类 号：R733.71[医药卫生—肿瘤]