益生屎肠球菌RS3的分离鉴定及增殖条件的优化  

Isolation and Identification of Probiotic Enterococcus faecium RS3 and Optimization of Its Proliferation Conditions

作　　者：刘玮 曹威 刘浩[2] 潘梅 LIU Wei;CAO Wei;LIU Hao;PAN Mei(Guilin Pharmaceutical Co.,Ltd.,Guilin 541004,Guangxi,China;Key Laboratory of Industrial Fermentation Microbiology,Ministry of Education,College of Biotechnology,Tianjin University of Science&Technology,Tianjin 300457,China)

机构地区：[1]桂林南药股份有限公司,广西桂林541004 [2]工业发酵微生物教育部重点实验室,天津科技大学生物工程学院,天津300457

出　　处：《食品研究与开发》2022年第13期156-163,共8页Food Research and Development

基　　金：国家自然科学基金青年科学基金项目(21706192)。

摘　　要：为筛选具有益生作用的乳酸菌,该研究从泡菜中筛选到1株细菌菌株RS3,经菌落形态观察、生化试验和16 S rRNA序列分析确定为屎肠球菌。为高效获得屎肠球菌RS3菌体,对其最佳增殖条件进行优化研究。利用单因素筛选和中心复合响应面试验对屎肠球菌RS3菌体增殖用液体培养基和增殖条件进行研究,优化的最佳培养基为乳糖15 g/L、胰蛋白胨25 g/L、初始p H值为8.2、培养温度37℃。与优化前培养条件相比,优化后培养条件下菌体生物量提高了54.9%,乳酸产量提高了53%,为后期规模化培养和益生菌剂开发奠定基础。To screen out lactic acid bacteria with probiotic effects,a bacterial strain RS3 was selected from kimchi and identified as Enterococcus faecium through colony morphology observation,biochemical experiment,and 16 S rRNA sequencing analysis.The optimal proliferation conditions were optimized to efficiently obtain E.faecium RS3 cells.Single-factor screening and central composite design in response surface methodology were applied to study the liquid medium and proliferation conditions for E.faecium RS3.The optimized medium contained 15 g/L lactose,25 g/L tryptone,10 g/L beef extract and 5 g/L sodium chloride,with initial pH 8.2,and the culture temperature was 37℃.Compared with results under pre-optimized culture conditions,the cell biomass under the optimized culture conditions increased by 54.9% and the lactic acid yield increased by 53%.This study was expected to lay the foundation for later large-scale culture and probiotic development.

关 键 词：益生菌 屎肠球菌 培养基 分离鉴定 增殖条件 

分 类 号：TS201.3[轻工技术与工程—食品科学]