工业大麻性别连锁Indel标记的筛选与鉴定  

Screening and Identification of Sex-linked Insertion-deletion (InDel) Markers in Industrial Hemp

作　　者：陶杰 潘根 黄思齐[1] 常丽 陈安国[1] 唐慧娟[1] 张翠萍[1] 李德芳[1] 李建军[1] 赵立宁[1] TAO Jie;PAN Gen;HUANG Siqi;CHANG Li;CHEN Anguo;TANG Huijuan;ZHANG Cuiping;LI Defang;LI Jianjun;ZHAO Lining(Institute of Bast Fiber Crops,Chinese Academy of Agricultural Sciences/Key Laboratory of Biological and Processing for Bast Fiber Crops,Ministry of Agriculture and Rural Affairs,Changsha 410205,Hunan,China)

机构地区：[1]中国农业科学院麻类研究所/农业农村部麻类生物学与加工重点实验室,湖南长沙410205

出　　处：《中国麻业科学》2022年第3期143-150,164,共9页Plant Fiber Sciences in China

基　　金：创新工程一年生麻类遗传改良团队(ASTIP-IBFC03);财政部和农业农村部:国家麻类产业技术体系资助(CARS-16-E-02)。

摘　　要：工业大麻为雌雄异株植株,少数为雌雄同株,其性别表达影响着工业大麻纤维和药用产量等经济价值。因此,为建立工业大麻早期性别快速筛选及鉴定的方法,研究利用InDel标记筛选出与工业大麻性别连锁的两组标记物(Is-02和Is-08),将其PCR产物通过聚丙烯酰胺电泳和1%琼脂糖凝胶电泳后,发现两组标记物分别在多个大麻种质中扩增出(370bp或272bp)雌性单一带型,雄性中呈现双带型(370bp和199bp,272bp和100bp),且带型清晰,重复性好,准确性高;在大麻雌雄异株种质、全雌品系和雌雄同株种质中都得到了验证,检测准确性可达100%。结果表明,Is-02和Is-08适用于工业大麻幼苗期未知性别、花期已知性别植株及品种的快速鉴定。研究可为工业大麻早期性别鉴定提供技术支持,也为工业大麻分子标记辅助育种提供新的分子标记手段。Most of hemp plants are dioecious, of which sex expression affects the economic value, such as fiber and medicinal yield. Therefore, in order to establish a rapid screening and identification method for early sex of hemp, InDel markers were used to screen out two groups of markers linked to sex of hemp(Is-02 and Is-08). Polyacrylamide electrophoresis and 1% agarose gel electrophoresis analysis showed that the two groups of markers amplified in multiple hemp germplasm(370 bp or 272 bp) with clear and high accuracy female single band type, male double band type(370 bp and 199 bp, 272 bp and 100 bp). It was verified in dioecious germplasm, all-female lines and monoecious germplasm of hemp with the detection accuracy of 100%. The results suggested that Is-02 and Is-08 were suitable for rapid identification of plants with unknown sex at seedling stage and known sex at flowering stage. This study could provide technical supports for early sex identification of hemp and new molecular markers for marker-assisted breeding of hemp.

关 键 词：工业大麻 性别连锁 INDEL标记 性别鉴定 

分 类 号：S563.3[农业科学—作物学]