人结肠癌5-氟尿嘧啶耐药细胞株SW620/Fu和LoVo/Fu的构建与鉴定  

Construction and Identification of 5-fluorouracil-resistant Human Colon Cancer Cell Lines SW620/Fu and LoVo/Fu

作　　者：张伟 朱壮彦[2] 张海燕[2] 韩丽炘 张建业 周雯敏 闫燕艳 ZHANG Wei;ZHU Zhuang-yan;ZHANG Hai-yan;HAN Li-xin;ZHANG Jian-ye;ZHOU Wen-min;YAN Yan-yan(The Second People's Hospital of Datong(Tumor Hospital),Datong Shanxi,037005;Medical School,Shanxi Datong University,Datong Shanxi,037009;Guangdong Provincial Key Laboratory of Molecular Target and Clinical Pharmacology,School of Pharmaceutical Sciences,Guangzhou Medical University,Guangzhou Guangdong,511436)

机构地区：[1]大同市第二人民医院(肿瘤医院),山西大同037005 [2]山西大同大学医学院,山西大同037009 [3]广州医科大学药学院,广东省分子靶标与临床药理学重点实验室,广东广州511436

出　　处：《山西大同大学学报（自然科学版）》2022年第3期59-62,共4页Journal of Shanxi Datong University(Natural Science Edition)

基　　金：国家自然科学基金资助项目[81902152];山西省卫健委“四个一批”重点专项[2020-XM33]。

摘　　要：目的构建耐5-氟尿嘧啶(5-fluorouracil,5-Fu)的人结肠癌SW620/Fu和LoVo/Fu细胞株,以建立稳定的体外实验模型。方法选取亲本人结肠癌细胞SW620和LoVo进行培养传代,通过药物逐步递增法建立体外5-Fu诱导的耐药细胞株,并对耐药细胞株进行短串重复序列(STR)分析;采用MTT法分别检测5-Fu对亲本株和耐药株的半数抑制浓度(IC_(50)),并计算5-Fu耐药指数(RI)。结果耐药细胞株与STR分析结果高度匹配,表明成功构建人结肠癌细胞5-Fu耐药细胞株SW620/Fu和LoVo/Fu。5-Fu对SW620和SW620/Fu细胞的IC_(50)值分别为58.17±3.54和443.71±14.27μmol/L;对LoVo和LoVo/Fu细胞的IC_(50)值分别为152.53±13.95和1049.54±70.51μmol/L(均P<0.05),SW620/Fu和LoVo/Fu细胞的耐药指数分别为7.63和6.88。结论药物逐步递增法成功构建了5-Fu耐药细胞株,为结肠癌耐药机制研究建立了平台,有利于逆转肿瘤耐药的研究。Objective To construct 5-fluorouracil(5-Fu)-resistant human colon cancer cell lines SW620/Fu and LoVo/Fu,and to establish a stable vitro experimental model.Methods The parental human colon cancer cells SW620 and LoVo were selected for culture and passage.5-Fu-resistant human colon cancer cell lines was established by step-by-step increasing the concentration of 5-Fu to parental SW620 and LoVo cells in vitro.Short-string repeat(STR)analysis of drug-resistant cell lines was carried out.The median inhibitory concentration(IC_(50))of 5-Fu against parental and resistant colon cancer cells was detected using MTT assay,and the 5-Fu resistance index(RI)was calculated.Results The drug-resistant cell lines were highly matched with the STR analysis results,indicating that the 5-Fu-resistant human colon cancer cell lines SW620/Fu and LoVo/Fu were successfully constructed.The IC_(50)values of 5-Fu in SW620 and SW620/Fu cells were 58.17±3.54 and 443.71±14.27μmol/L,respectively;the IC_(50)values of 5-Fu in LoVo and LoVo/Fu cells were 152.53±13.95 and 1049.54±70.51μmol/L,respectively(all P<0.05).The RI of the SW620/Fu and LoVo/Fu were7.63 and 6.88,respectively.Conclusion The 5-Fu-resistant cell line was successfully constructed by the drug step-by-step method,which established a platform for the study of the drug resistance mechanism of colon cancer,and was beneficial to the research on reversing the tumor resistance.

关 键 词：结肠癌 5-氟尿嘧啶(5-Fu) 肿瘤耐药 构建 鉴定 

分 类 号：R73-3[医药卫生—肿瘤]