EAE小鼠脑和脊髓维生素D受体蛋白表达差异研究  

Expression of vitamin D receptor protein in brain and spinal cord of experimental autoimmune encephalomyelitis

作　　者：朱俊羽 楚兰[1] 徐竹 高子丹 丹珍卓玛 ZHU Junyu;CHU Lan;XU Zhu(Guizhou Medical University,Guiyang 550004,China)

机构地区：[1]贵州医科大学,贵州贵阳550004 [2]贵州医科大学附属医院神经内科,贵州贵阳550004

出　　处：《中风与神经疾病杂志》2022年第5期419-424,共6页Journal of Apoplexy and Nervous Diseases

基　　金：国家自然科学基金资助项目(No.81960231)。

摘　　要：目的探讨在实验性自身免疫性脑脊髓炎(EAE)小鼠和健康对照小鼠不同中枢神经系统区域和在不同神经功能评分时维生素D受体(VDR)表达量的差异。方法按Weaver’s 15分神经功能评分法将EAE小鼠分为0分组、高分组、低分组,采用透射电子显微镜观察各组小鼠髓鞘结构;采用Western Blot测定EAE高分组小鼠和健康对照组小鼠皮质、白质、小脑、脑干、脊髓组织的VDR蛋白表达量;根据结果,选择在脊髓组织进一步对健康对照组与EAE各组间进行VDR蛋白表达量的比较。结果在透射电子显微镜下观察EAE 0分组、高分组、低分组小鼠髓鞘结构,对比健康对照组都有不同程度的破坏:0分组、低分组髓鞘环形结构存在,高分组髓鞘环形结构消失。在皮质、白质、小脑、脑干、脊髓组织中,EAE组的VDR蛋白表达均较健康对照组低(皮质P=0.893;白质P=0.767;小脑P=0.225),其中脑干和脊髓的VDR蛋白表达明显低于健康对照组(P=0.046;P=0.009);在脊髓组织中,EAE 0分组、高分组、低分组VDR蛋白表达都低于健康对照组(P=0.005;P=0.009;P<0.0001);高分组较0分组低(P=0.716),低分组较0分组进一步下降(P=0.003),同时低分组较高分组也呈下降趋势(P=0.013)。结论EAE小鼠不同中枢神经系统区域VDR蛋白表达有差异,不同神经功能评分组VDR蛋白表达有差异。Objective To investigate the expression of vitamin D receptor(VDR)in different central nervous system regions and neurological function scores of experimental autoimmune encephalomyelitis(EAE)mice and healthy control mice.Methods EAE mice were divided into 0 score group,high score group and low score group according to Weaver’s 15 score neurological function score.The myelin sheath structure of each group was observed by transmission electron microscope.And Western Blot was used to detect the expression of VDR protein in cortex,white matter,cerebellum,brain stem and spinal cord of EAE high score group mice and healthy control group mice.According to the results,the expression of VDR protein in spinal cord was further compared between healthy control group and EAE groups.Results The myelin sheath structure of 0 score group,high score group and low score group mice was observed under transmission electron microscope.Compared with the healthy control group,the myelin sheath ring structure of 0 group and low group existed,and the myelin sheath ring structure of high score group disappeared.In cortex,white matter,cerebellum,brainstem and spinal cord,the expression of VDR protein in EAE group was lower than that in healthy control group(P cortex=0.893;P white matter=0.767;Pcerebellum=0.225).The expression of VDR protein in brainstem and spinal cord was significantly lower than that in healthy control group(P=0.046;P=0.009).In spinal cord,the expression of VDR protein in 0 score group,high score group and low score group was lower than that in healthy control group(P=0.005,P=0.009,P<0.0001);high score group was lower than 0 score group(P=0.716),low score group was further lower than 0 score group(P=0.003),while low score group and high score group also showed a downward trend(P=0.013).Conclusions The expression of VDR protein in different central nervous system regions of EAE mice was different,and the expression of VDR protein was different in different nerve function score groups.

关 键 词：维生素D受体 实验性自身免疫性脑脊髓炎 多发性硬化 小鼠 

分 类 号：R744.51[医药卫生—神经病学与精神病学]