急性脑梗死中血小板反应蛋白1表达与血管生成因子的相关关系  

The relationship between thrombospondin 1 expression and angiogenic factors in acute cerebral infarction

作　　者：李静娜 张斯淼 程丹 姚玉兰 王运良[1,2] LI Jingna;ZHANG Simiao;CHENG Dan(Department of Neurology,Second Affiliated Hospital of Zhengzhou University,Zhengzhou 450014,China)

机构地区：[1]郑州大学第二附属医院神经内科,河南郑州450014 [2]中国人民解放军960医院神经内科,山东淄博255300

出　　处：《中风与神经疾病杂志》2022年第5期438-441,共4页Journal of Apoplexy and Nervous Diseases

基　　金：河南省卫生计生科技创新型人才“51282”工程资助项目(豫卫科[2016]32号)。

摘　　要：目的探讨在急性脑梗死中血小板反应蛋白1(TSP1)与血管生成因子之间的关系。方法选取郑州大学第二附属医院神经内科107例急性脑梗死作为病例组,同期入院体检的健康者90例作为对照组。采用酶联免疫方法(ELISA)进行检测TSP1、转化生长因子(TGFα)、成纤维细胞生长因子2(FGF2),比较病例组与对照组三者表达情况,以及不同NIHSS评分下三者表达情况;利用Spearman方法分析TSP1与TGFα、FGF2之间的相关性;多因素回归法分析脑梗死发生的危险因素;ROC曲线分析TSP1、TGFα和FGF2对脑梗死的预后情况。结果病例组TSP1、TGFα、FGF2均高于对照组,差异有统计学意义(P<0.05);NIHSS评分分组仅TSP1差异有统计学意义(P<0.05),且轻度组<中度组<重度组;TSP1表达水平与TGFα、FGF2成正相关(P<0.05);回归分析结果示TSP1、TGFα、FGF2是脑梗死的危险因素;ROC曲线示三者均可作为脑梗死疾病诊断的潜在指标。结论病例组TSP1作为血管生成的抑制剂与促进血管生成因子相互作用,可能是急性脑梗死患者预后不良的因素。Objective To investigate the relationship between thrombospondin 1(TSP1)and angiogenesis factors in acute cerebral infarction.Methods 107 cases of acute cerebral infarction in the Department of Neurology,the Second Affiliated Hospital of Zhengzhou University were selected as the case group,and 90 healthy people admitted to hospital for physical examination were selected as the control group.Enzyme-linked immunosorbent assay(ELISA)was used to detect TSP1,transforming growth factor(TGFα)and fibroblast growth factor 2(FGF2),and the expressions of the three were compared between the case group and the control group,as well as under different NIHSS scores;Spearman method was used to analyze the correlation between TSP1 and TGFα,FGF2;multivariate regression method was used to analyze the occurrence of cerebral infarction The risk factors of cerebral infarction;ROC curve analysis of TSP1,TGFαand FGF2 on the prognosis of cerebral infarction.Results TSP1,TGFα,FGF2 in the case group were higher than those in the control group,and the difference was statistically significant(P<0.05).NIHSS score grouping only had significant difference in TSP1(P<0.05),and mild group<moderate group<severe group.The expression level of TSP1 was positively correlated with TGFαand FGF2(P<0.05).It is a risk factor for cerebral infarction;ROC curve shows that all three can be used as potential indicators for the diagnosis of cerebral infarction.Conclusion TSP1 in the case group acts as an angiogenesis inhibitor and interacts with angiogenesis-promoting factors,which may be a poor prognosis factor in patients with acute cerebral infarction.

关 键 词：急性脑梗死 血小板反应蛋白1 转化生长因子Α 成纤维细胞生长因子2 

分 类 号：R743.3[医药卫生—神经病学与精神病学]