不同矮化中间砧对华红苹果果实中总酚及组分含量的影响  

Effects of different dwarfing interstocks on the total phenols and polyphenol components in Huahong apple

作　　者：李青山 高源[2] 王昆[2] 孙思邈 路翔 李连文[2] 冯建荣[1] 王大江[2] LI Qingshan;GAO Yuan;WANG Kun;SUN Simiao;LU Xiang;LI lianwen;FENG Jianrong;WANG Dajiang(College of Agriculture,Shihezi University/The Key Laboratory of Special Fruits and Vegetables Cultivation Physiology and Germplasm Resources Utilization,Xinjiang Production and Construction Corps,Shihezi 832000,Xinjiang,China;Research Institute of Pomology,Chinese Academy of Agricultural Sciences/Key Laboratory of Horticultural Crops Germplasm Resources Utilization,Ministry of Agriculture and Rural Affairs,Xingcheng 125100,Liaoning,China)

机构地区：[1]石河子大学农学院·特色果树栽培生理与种质资源利用兵团重点实验室,新疆石河子832000 [2]中国农业科学院果树研究所·农业农村部园艺作物种质资源利用重点实验室,辽宁兴城125100

出　　处：《果树学报》2022年第7期1191-1202,共12页Journal of Fruit Science

基　　金：中国农业科学院科技创新工程项目(CAAS-ASTIP-2018-RIP-02);财政部和农业农村部:国家现代农业产业技术体系资助项目(CARS-27)。

摘　　要：【目的】探讨6个不同矮化中间砧对华红苹果果实中总酚及组分动态含量的影响,为苹果矮化中间砧的选择应用和优质苹果生产提供应用依据。【方法】以山荆子为基砧,CG24、MD001、SH3、SH38、Mac9和CX5为中间砧,嫁接华红为试验材料,山荆子直接嫁接华红为对照;在果实生长发育的7个时期,采用超高效液相色谱法及质谱联用技术测定多酚组分及含量、Folin-Ciocalteus法测定总酚含量。【结果】华红果皮中总酚含量在所有时期均高于果肉,且随着果实的发育逐渐降低,但在发育过程中出现最小值的时期不同:果肉在成熟期最低,果皮8月29日最低;果皮、果肉多酚组分含量随着果实发育和成熟逐渐降低或在果实发育阶段先降低,随着果实成熟增高,不同矮化中间砧组合对果皮、果肉多酚组分的动态变化有不同程度的影响。在成熟期,SH38组合、CG24组合和SH3组合提高了华红果皮总酚含量,MD001组合和SH38组合提高了华红果肉总酚含量;Mac9组合和SH38组合显著提高华红花青苷含量,进而促进果实着色;SH38组合和MD001组合对果皮、果肉多酚组分的含量提升较多。【结论】SH38组合可考虑作为提高华红苹果功能性栽培的首选中间砧。【Objective】The dynamic effects of six different dwarfing interstocks on the total phenols and polyphenol components in Huahong apple were studied in order to provide a basis for selection and application of apple dwarf interstocks and fruit production with high quality.【Methods】The rootsock was Malus baccata(L.)Borkh.,and the interstocks were CG24,SH38,SH3,MD001,Mac9 and CX5.The scion variety Huahong was grafted on the interstocks,and the trees of Huahong grafted on M.baccata(L.)Borkh.were used as control(CK).The components and contents of polyphenols in the peel and flesh of Huahong fruits were analyzed at seven periods during Huahong apple growth using High Efficiency Liquid Chromatography and Mass Spectrometry,and the content of total phenols was determined by Folin-Ciocalteu method.【Results】The content of total phenols in the peel of Huahong fruits was significantly higher than that in the flesh,the content of total phenols in the peel and flesh decreased gradually during the period of the fruit development.However,the periods of the lowest content of total phenols were different in the peel and flesh,it was the 8thOctober in the flesh and the 29;August in the peel.The content of polyphenols in the peel and flesh decreased gradually with fruit development and maturity or decreased first at the stage of fruit development,and then increased at the maturity stage.Different interstocks had different effects on the dynamic changes of polyphenols in the peel and flesh.At maturity stage,the content of total phenols in the peel of Huahong fruits from high to low was SH38>CG24>SH3>CK>MD001>CX5>MAC9,and the content of total phenols in the flesh was MD001>SH38>CK>CX5>CG24>MAC9>SH3.During the period of fruit ripening,SH3,SH38 and Mac9 significantly increased the content of flavonols in the peel,MD001significantly increased the content of hydroxycinnamic acids in the flesh.The content of quercetin-3-alactoside was the highest in the peel and the content of procyanidin B2 was the highest in the flesh at the sta

关 键 词：苹果 华红 矮化中间砧 总酚 多酚组分 

分 类 号：S661.1[农业科学—果树学]