百合育子方治疗弱精子症及其对精子CFTR表达的影响  

Baihe Yuzi Prescription improves asthenospermia by up-regulating the expression of CFTR in the sperm

作　　者：李波[1] 陈广辉[1] 王广建 申玉行 邓晋超 葛建立[1] 杨德华[1] LI Bo;CHEN Guang-hui;WANG Guang-jian;SHEN Yu-hang;DENG Jin-chao;GE Jian-li;YANG De-hua(Department of Andrology,Hebei Provincial Hospital of Traditional Chinese Medicine,Shijiazhuang,Hebei 050011,China;Department of Surgery,Nankou Hospital of Changping District,Changpin,Beijing 102200,China;Graduate School,Hebei College of Traditional Chinese Medicine,Shijiazhuang,Hebei 050091,China)

机构地区：[1]河北省中医院男科,河北石家庄050011 [2]北京市昌平区南口医院外科,北京102200 [3]河北中医学院研究生学院,河北石家庄050091

出　　处：《中华男科学杂志》2022年第5期432-436,共5页National Journal of Andrology

基　　金：国家自然科学基金(81804108);河北省重点研发计划项目(19277705D)。

摘　　要：目的:观察百合育子方治疗无证可辨弱精子症的临床疗效及其对精液参数、精子形态及DNA碎片指数(DFI)、精子囊性纤维化跨膜传导调节因子(CFTR)表达的影响。方法:112例临床无证可辨的弱精子症患者随机分为试验组(57例)和对照组(55例),分别服用百合育子方和左卡尼汀口服液联合维生素E软胶囊治疗3个月,观察两组的疗效及治疗前后精子总数、活动率、前向运动精子百分率、正常形态精子百分率及DFI、精子CFTR表达的变化。结果:试验组总有效率为82.46%,优于照组的65.45%(P<0.05);与治疗前相比,试验组患者的精子总数[(53.5±3.5)×10^(6) vs(86.5±3.9)×10^(6)]、活动率[(23.5±3.5)%vs(38.8±3.7)%]、前向运动精子百分率[(20.1±3.2)%vs(30.3±3.3)%]、正常形态精子百分率[(2.3±0.3)%vs(3.9±0.4)%]、精子DFI[(37.3±3.1)%vs(25.2±3.4)%]、CFTR表达量均显著性改善(P<0.05);对照组患者精子总数、精子CFTR表达量变化无统计学意义(P>0.05);活动率[(23.8±3.7)%vs(30.2±3.4)%]、前向运动精子百分率[(19.6±3.1)%vs(25.3±2.9)%]、正常形态精子百分率[(2.4±0.4)%vs(3.1±0.3)%]、精子DFI[(36.6±3.3)%vs(30.3±3.1)%]显著性改善(P<0.05),且试验组均优于对照组,差异有统计学意义(P<0.05)。结论:百合育子方可提高弱精子症患者的精子总数和活力,改善精子形态和DFI,其对精子活力、DFI的影响可能与提高精子CFTR表达有关。Objective:To observe the therapeutic efficacy of Baihe Yuzi Prescription(BYP)in the treatment of clinical syndrome-free asthenospermia and its effects on semen parameters,sperm DNA fragmentation index(DFI)and the expression of the cystic fibrosis transmembrane conductance regulator(CFTR)in the sperm.Methods:We randomly divided 112 patients with clinical syndrome-free asthenospermia into a control group(n=55)and an experimental group(n=57),the former treated orally with L-carnitine liquid combined with vitamin E capsules and the latter with BYP in addition,both for 3 months.After treatment,we obtained the total sperm count,sperm motility,percentages of progressively motile sperm(PMS)and morphologically normal sperm(MNS),sperm DFI and expression of CFTR in the sperm,and compared the above parameters between the two groups of patients before and after medication.Results:The total effectiveness rate was significantly higher in the experimental group(82.46%)than in the control(65.45%)(P<0.05).Compared with the baseline,the patients in the experimental group showed significant improvement after treatment in the total sperm count([53.5±3.5]vs[86.5±3.9]×10^(6),P<0.05),sperm motility([23.5±3.5]%vs[38.8±3.7]%,P<0.05),PMS([20.1±3.2]%vs[30.3±3.3]%,P<0.05),MNS([2.3±0.3]%vs[3.9±0.4]%,P<0.05),sperm DFI([37.3±3.1]%vs[25.2±3.4]%,P<0.05)and the expression of CFTR(P<0.05),and even better improvement than the controls in sperm motility([23.8±3.7]%vs[30.2±3.4]%,P<0.05),PMS([19.6±3.1]%vs[25.3±2.9]%,P<0.05),MNS([2.4±0.4]%vs[3.1±0.3]%,P<0.05),and sperm DFI([36.6±3.3]%vs[30.3±3.1]%,P<0.05).The total sperm count and the expression of CFTR,however,were not significantly improved in the control group after treatment(P>0.05).Conclusion:Baihe Yuzi Prescription can increase sperm count and motility,improve sperm morphology and DFI in patients with clinical syndrome-free asthenospermia,which may be related to the up-regulated expression of CFTR in the sperm.

关 键 词：百合育子方 弱精子症 无证可辨 补肺益肾 囊性纤维化跨膜传导调节因子 

分 类 号：R698.2[医药卫生—泌尿科学]