2011和2019年甘肃省库蚊黄病毒分子遗传进化特征  

Molecular evolution of Culex flavivirus in Gansu province in 2011 and 2019

作　　者：李彬 查冰[2] 付士红[2] 徐学平 应思超 聂凯[2] 李兴洲[1] 王环宇[2] 梁国栋[2] Li Bin;Zha Bing;Fu Shihong;Xu Xueping;Ying Sichao;Nie Kai;Li Xingzhou;Wang Huanyu;Liang Guodong(School of Public Health,Jiamusi University,Jiamusi 154007,China;Department of Arboviruses,National Institute for Viral Disease Control and Prevention,State Key Laboratory for Infectious Disease Prevention and Control,Chinese Center for Disease Control and Prevention,Beijing 100052,China)

机构地区：[1]佳木斯大学公共卫生学院,佳木斯154007 [2]中国疾病预防控制中心病毒病预防控制所虫媒室传染病预防控制国家重点实验室,北京100052

出　　处：《中华实验和临床病毒学杂志》2022年第2期183-188,共6页Chinese Journal of Experimental and Clinical Virology

基　　金：传染病预防控制国家重点实验室重点项目(2015SKLID505)。

摘　　要：目的揭示2011年和2019年甘肃蚊虫中库蚊黄病毒的分子遗传差异。方法使用逆转录聚合酶链反应(reverse transcriptase-polymerase chain reaction,RT-PCR)获得甘肃2011年和2019年蚊虫中库蚊黄病毒基因组核苷酸序列,利用病毒分子生物学和生物信息学方法分析病毒基因差异。结果共获得10株库蚊黄病毒基因核苷酸序列,包括2011年8株(均来自于淡色库蚊)、2019年2株(来自三带喙库蚊和中华按蚊)。病毒E基因核苷酸与氨基酸序列同源性分析结果显示,甘肃2019年分离的病毒与2011年分离病毒核苷酸序列相似性98.4%~100%,氨基酸相似性95.4%~97.3%。库蚊黄病毒E基因序列系统进化分析结果显示,2019年甘肃分离的2株库蚊黄病毒(GS1975和GS1976)与2011年的8株库蚊黄病毒均属于基因1型B组库蚊黄病毒;2019年分离的GS1975病毒与我国辽宁(2010年和2011年)和内蒙(2018年)分离病毒处在共同的进化簇,而2019年分离的GS1976病毒与我国内蒙(2018年)和甘肃2011年分离病毒形成共同进化簇。结论甘肃2011年和2019年分离的库蚊黄病毒均属于基因1型病毒,2019年分离的2株病毒分布在不同的进化簇,随着时间的推移当地库蚊黄病毒基因组在发生变化,为此需要开展当地库蚊黄病毒分子差异的长期监测。Objective To disclose the molecular genetic differences of Culex flavivirus among mosquitoes in Gansu province in 2011 and 2019.Methods Reverse transcriptase-polymerase chain reaction(RT-PCR)was used to obtain the nucleotide sequences of Culex flavivirus genomes from mosquitoes in Gansu province in 2011 and 2019,and viral molecular biology and bioinformatics method were used to analyze the genetic differences of the viruses.Results Nucleotide sequences of 10 strains of Culex flavivirus were obtained,including 8 strains(all from Culex pipiens pallens)obtained in 2011 and 2 strains(from Culex tritaeniorhynchus and Anopheles sinensis)in 2019.Homology analysis of nucleotide and amino acid sequences of virus E gene showed that the nucleotide sequence similarity and amino acid similarity of viruses isolated from Gansu in 2019 and 2011 ranged from 98.3%-100%and 95.4%-97.3%,respectively.Phylogenetic analysis of Culex flavivirus E gene sequence showed that two strains of Culex flavivirus isolated in Gansu province in 2019(GS1975 and GS1976)and eight strains of Culex flavivirus isolated in 2011 all belonged to group B of genotype 1 of Culex flavivirus.Further analysis found that GS1975 virus isolated in 2019 was in a common evolutionary cluster with viruses isolated from Liaoning(2010 and 2011)and Inner Mongolia(2018),while GS1976 virus isolated in 2019 formed a coevolutionary cluster with viruses isolated from Inner Mongolia(2018)and Gansu(2011).Conclusions Although both Culex flaviviruses isolated in Gansu province in 2011 and 2019 are genotype 1 virus,the two viruses isolated in 2019 distributed in two different evolutionary clusters,suggesting that the local mosquito virus genome changes over time,therefore,long-term monitoring of molecular differences is needed to carry out.

关 键 词：库蚊 黄病毒 系统进化分析 

分 类 号：R373.3[医药卫生—病原生物学]