广东省1例原发性免疫缺陷患者排出的Ⅱ型疫苗衍生脊髓灰质炎病毒分子病毒学特征  

Molecular virological characteristics of type 2 vaccine-derived polioviruses excreted from a primary immunodeficiency patient in Guangdong province

作　　者：陈美钟 张薇[2] 黄淑芬 郭雪[2] 祝双利[3] 曾汉日[2] 严冬梅[3] 方苓[2] 张勇[3] 郑焕英[2] 邓小玲 Chen Meizhong;Zhang Wei;Huang Shufen;Guo Xue;Zhu Shuangli;Zeng Hanri;Yan Dongmei;Fang Ling;Zhang Yong;Zheng Huanying;Deng Xiaoling(School of Public Health,Southern Medical University,Guangzhou 510515,Guangdong,China;Guangdong Workstation for Emerging Infectious Disease Control and Prevention,Guangdong Provincial Center for Disease Control and Prevention,Guangzhou 511430,Guangdong,China;WHO WPRO Regional Reference Laboratory for Poliomyelitis/National Health Commission Key Laboratory for Biosafety/National Institute for Viral Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206,China)

机构地区：[1]南方医科大学公共卫生学院,广东广州510515 [2]广东省疾病预防控制中心广东新发突发传染病防治工作站,广东广州511430 [3]世界卫生组织西太平洋区脊髓灰质炎参比实验室,国家卫生健康委员会生物安全重点实验室,中国疾病预防控制中心病毒病预防控制所,北京102206

出　　处：《中国疫苗和免疫》2022年第3期263-267,共5页Chinese Journal of Vaccines and Immunization

基　　金：广东省医学科学技术研究基金项目(B2022156)。

摘　　要：目的分析1例原发性免疫缺陷(Primary immunodeficiency,PID)患者持续排出的Ⅱ型免疫缺陷疫苗衍生脊髓灰质炎(脊灰)病毒(Immunodeficiency vaccine-derived poliovirus,iVDPV)分子病毒学特征。方法对2015年广东省云浮市1例患PID的急性弛缓性麻痹病例连续采集粪便标本并进行脊灰病毒分离,使用逆转录-聚合酶链反应对分离株VP1编码区进行扩增和核苷酸序列测定,分析核苷酸和氨基酸相似性。结果2015年3-10月从该例PID患者分离出13株Ⅱ型脊灰病毒,包括8株iVDPV和5株Sabin疫苗株。13株分离株VP1区与Ⅱ型Sabin疫苗参考株的核苷酸相似性为99.1%-99.6%;随着排毒时间延长,核苷酸和氨基酸变异数量增加,其中I143V和Y185F是所有分离株共享的非同义突变。结论本研究PID患者排出的iVDPV存在共同的初始进化祖先,在患者体内持续复制并发生多种突变。ObjectiveTo analyze molecular virological characteristics of type 2 immunodeficiency vaccinederived polioviruses( i VDPV) excreted from an individual with a primary immunodeficiency( PID).MethodsWe collected fecal samples periodically from an acute flaccid paralysis case with PID in Yunfu city, Guangdong province in 2015 and isolated polioviruses from the samples. We amplified and sequenced the VP1 coding region of isolates using reverse transcription-polymerase chain reaction to determine nucleotide and amino acid similarities.ResultsThirteen strains of poliovirus type 2 were isolated from the PID patient from March to October 2015;8 were i VDPV strains and 5 were Sabin-like strains. Compared with type 2 Sabin vaccine reference strain, nucleotide similarity in the VP1 coding regions of the 13 isolates was 99. 1%-99. 6%. Nucleotide and amino acid mutations increased over time;I143V and Y185F were non-synonymous mutations in all isolates.ConclusionsThe i VDPVs excreted from this PID patient had a common evolutionary ancestor and continuously replicated, accruing mutations.

关 键 词：脊髓灰质炎 疫苗衍生脊髓灰质炎病毒 原发性免疫缺陷 分子病毒学特征 

分 类 号：R186[医药卫生—流行病学]