固定化酶催化抗坏血酸硬脂酸酯的合成及抗氧化活性  

Synthesis and Antioxidant Activity of Ascorbic Acid Stearate by Immobilized Enzyme

作　　者：王彬 孙尚德[1] 陈晓中 WANG Bin;SUN Shangde;CHEN Xiaozhong(College of Food Science and Technology,Henan University of Technology,Zhengzhou 450052,China)

机构地区：[1]河南工业大学粮油食品学院,河南郑州450052

出　　处：《食品与生物技术学报》2022年第6期98-105,共8页Journal of Food Science and Biotechnology

基　　金：全国粮食行业青年拔尖人才项目(LQ2020101);河南省高等学校重点科研项目计划基础研究专项(21zx010)。

摘　　要：研究液体脂肪酶CALB的固定化及其催化抗坏血酸和硬脂酸的酯化反应合成抗坏血酸硬脂酸酯(AS)。以大孔吸附树脂为固定化载体,对液体脂肪酶CALB进行固定,固定化负载率可达72%。探讨不同反应变量(反应溶剂、温度、酶添加量、底物物质的量比、底物浓度和分子筛添加量)对酯化反应的影响,并对反应条件进行优化,优化的反应条件为:反应温度72℃,底物物质的量比1:12,酶添加量22.49%(质量分数),反应24 h,在此优化条件下,AS产率可达(41.33±1.81)%。对产品进行分离纯化,并采用质谱等检测确定产品为AS。通过Rancimat实验发现AS具有良好的抗氧化性,添加100 mg/kg AS的玉米油氧化诱导期与添加50 mg/kg TBHQ的玉米油氧化诱导期接近。The immobilization of liquid lipase CALB was investigated,which was used to catalyze the esterification of L-ascorbic acid with stearic acid to prepare L-ascorbic acid stearate(AS).The macroporous adsorbent resin was used as the immobilized carrier for liquid lipase CALB,and the immobilization loading rate was up to 72%.The effect of different reaction variables(reaction solvent,temperature,enzyme loading amount,molar ratio of substrate,substrate concentration and molecular sieve addition amount)on the esterification was investigated and the reaction conditions were optimized.The optimal conditions were 72℃,1∶12 molar ratio of L-ascorbic acid and stearic acid,22.49%enzyme loading amount(mass fraction),and 24 h of reaction.Under the optimal reaction conditions,the maximum yield of AS was(41.33±1.81)%.The product was separated and purified,and the product was identified as AS by mass spectra.The results of Rancimat showed that AS had good antioxidant activity,and the oxidation induction period of corn oil with 100 mg/kg AS was similar to that with 50 mg/kg of TBHQ.

关 键 词：CALB固定化 酶催化合成 抗坏血酸硬脂酸酯 响应面法 RANCIMAT法 

分 类 号：TL271.5[核科学技术—核燃料循环与材料]