运动强度与高脂饮食模型大鼠脂代谢紊乱和氧化应激的相关性  

Correlation between exercise intensity and lipid metabolism disorder and oxidative stress in a highdiet rat model

作　　者：阮凌 王光华 吴荣平 晋战 吕镇庆 张楠 李寿邦[1] Ruan Ling;Wang Guanghua;Wu Rongping;Jin Zhan;Lyu Zhenqing;Zhang Nan;Li Shoubang(Department of Physical Education,Xi’an Shiyou University,Xi’an 710065,Shaanxi Province,China)

机构地区：[1]西安石油大学体育系,陕西省西安市710065

出　　处：《中国组织工程研究》2023年第8期1149-1155,共7页Chinese Journal of Tissue Engineering Research

基　　金：陕西省自然科学基础研究计划项目(2021JQ-586),项目名称:运动通过调控细胞自噬实现对NAFLD的保护及其机制研究,项目负责人:阮凌。

摘　　要：背景:肥胖是一种多因素的慢性疾病,经常运动可以改善肥胖群体脂代谢紊乱和氧化应激的现象,但不同强度运动对脂肪代谢的具体机制尚不清楚。目的:探讨不同强度运动对肥胖大鼠脂代谢和氧化应激等指标的影响,并进一步阐明过氧化物酶体增殖物激活受体α、肝X受体α及相关通路蛋白与脂代谢指标的相关性及具体作用机制。方法:将大鼠随机分为5组:即正常对照组(n=10)、肥胖模型组(n=10)、小强度运动组(n=10)、中等强度运动组(n=9)和大强度运动组(n=8),正常对照组给予普通饲料,后4组均喂饲高脂饮食,喂养16周制备肥胖大鼠模型,造模成功后各运动组每周运动5 d,每次1 h。运动6周后测试大鼠体质量、肝质量、心脏质量、体脂率、血脂、脂酶和氧化应激指标,脂肪组织病理切片观察脂肪细胞的形态和结构;用Western blot法测定肝脏中载脂蛋白A5、过氧化物酶体增殖物激活受体α、固醇调节元件结合蛋白1c和肝X受体α的蛋白表达水平。结果与结论:①与肥胖模型组相比,中等强度运动显著降低肥胖大鼠三酰甘油、低密度脂蛋白和游离脂肪酸水平(P<0.01),同时显著升高脂蛋白脂酶和肝脂酶活性(P<0.05);②各运动组中血浆丙二醛水平显著降低(P<0.01),而过氧化氢酶活性显著升高(P<0.01);③各运动组载脂蛋白A5蛋白表达水平均显著升高(P<0.05,P<0.01,P<0.01),固醇调节元件结合蛋白1c和肝X受体α的表达均降低(P<0.01),而过氧化物酶体增殖物激活受体α水平仅在中等强度运动组显著升高(P<0.05);④与小强度运动组和大强度运动组相比,中等强度运动组载脂蛋白A5水平显著升高(P<0.01,P<0.05);而与小强度运动组相比,中强度运动组固醇调节元件结合蛋白1c表达水平显著降低(P<0.01);⑤提示不同强度运动对肥胖大鼠脂代谢相关指标和氧化应激指标均有改善,中等强度运动依赖过氧化物酶体增殖物激活�BACKGROUND:Obesity is a multifactorial chronic disease.Regular exercise can improve lipid metabolism disorders and oxidative stress in obese groups,but the specific exercise intensity is unclear.OBJECTIVE:To explore the effect of different-intensity exercises on lipid metabolism and oxidative stress in obese rats and to clarify the correlation of peroxisome proliferators-activated receptorα,liver X receptorαand related pathway proteins with lipid metabolism as well as the specific mechanism.METHODS:Rats were randomly divided into five groups:normal control group(n=10),obesity model group(n=10),low-intensity exercise group(n=10),moderate-intensity exercise group(n=9)and high-intensity exercise group(n=8).The normal control group was given ordinary feed,and the other four groups were fed with high-fat diet for 16 weeks to prepare the obese rat model.After modelling,rats in each group were arranged to run on the treadmill 1 hour per day and 5 days per week.After 6 weeks of training,body mass,liver mass,heart mass,and body fat percentage,lipids,lipases and oxidative stress were tested,and adipose tissue were cut into sections for cell morphological and structural observations.The protein expression of apolipoprotein A5,peroxisome proliferators-activated receptorα,sterol regulatory element-binding protein 1c and liver X receptorαin liver tissue were detected by western blot assay.RESULTS AND CONCLUSION:Compared with the obesity model group,the levels of triacylglycerols,low-density lipoproteins,and free fatty acids reduced were significantly reduced,while the activities of lipoprotein lipase and hepatic lipase increased significantly in the moderate-intensity exercise group(P<0.01).Plasma malondialdehyde level showed a significant reduction in each exercise group(P<0.01),but the activity of catalase increased significantly in all exercise groups(P<0.01).Compared with the obesity model group,the protein expression of apolipoprotein A5 was significantly increased in each exercise group(P<0.05,P<0.01,P<0.01)and the e

关 键 词：运动 肥胖 运动强度 高脂饮食 脂代谢 氧化应激 动物模型 

分 类 号：R459.9[医药卫生—治疗学] R318[医药卫生—临床医学] R875