布氏轮藻肌动蛋白基因家族的全基因组鉴定及表达分析  

Wholegenome identification and expression analysis of actin gene family in Chara braunii

作　　者：胡莎莎 张冬群 张苡芃 韩鹏举 兰利琼[1] 卿人韦[1] HU Sha-Sha;ZHANG Dong-Qun;ZHANG Yi-Peng;HAN Peng-Ju;LAN Li-Qiong;QING Ren-Wei(Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education,College of Life Sciences,Sichuan University?Chengdu 610065,China)

机构地区：[1]四川大学生命科学学院生物资源与生态环境教育部重点实验室,成都610065

出　　处：《四川大学学报（自然科学版）》2022年第4期173-180,共8页Journal of Sichuan University(Natural Science Edition)

基　　金：国家自然科学基金(30470143)。

摘　　要：为了解肌动蛋白actin在轮藻植物生长发育中的潜在功能,本研究利用生物信息学方法对布氏轮藻肌动蛋白基因家族进行了鉴定及表达分析.在布氏轮藻中共鉴定出16个肌动蛋白基因,将其重命名为CbACT1~CbACT16,其氨基酸长度为361~1182 AA,相对分子质量为39 886.71~117 256.72 Da,等电点介于4.68~8.93;亚细胞定位预测显示15个肌动蛋白基因位于细胞质中,1个位于叶绿体中;二级结构结果表明肌动蛋白基因主要以无规则卷曲和α螺旋为主;共线性分析中仅发现一对源自片段重复的旁系同源基因;系统发育结果表明轮藻肌动蛋白基因可以分为两个亚家族,结合基因结构及保守基序分析,同一亚家族倾向于具有相似的外显子分布,较多的共有基序;基于启动子顺势作用元件分析表明,16个肌动蛋白基因参与了许多与生物和非生物胁迫、激素调节和光反应有关的生命活动.组织表达分析显示,16个基因在四种不同组织中存在差异性表达,表明它们在不同组织的生长发育过程中具有不同的功能.因此,轮藻肌动蛋白基因家族可能参与了轮藻植物不同组织的发育过程及响应生物和非生物胁迫等的过程.In order to understand the potential functions of actin in the growth and development of Charophytes, bioinformatics methods were used to analyze the actin gene family in Chara braunii. A total of 16 actin genes were identified in Chara braunii, and they were renamed as CbACT1~CbACT16 according to their order on scaffolds with 361 to 1182 amino acids, and the relative molecular masses ranged form 39 886.71 to 117 256.72 Da, and protein isoelectric point was range from 4.68 to 8.93. Subcellular localization prediction indicated that 15 actin genes were located in the cytoplasm;and only one in the chloroplast. The secondary structure of the actin gene was mainly composed of random coils and α-helices. Only one pair of paralogous genes derived from segmental duplication was found in the collinearity analysis. The phylogenetic results divided actin genes into two subfamilies with the same subfamily tend to have similar exon distribution and more common motifs. Promoter cis-element analysis indicated that 16 actin genes were involved in many biological activities including biotic and abiotic stresses, hormone regulation and light response. Further tissue expression analysis suggested that 16 genes were differentially expressed in four different tissues, indicating that they have different functions in the growth and development of different tissues. Therefore, the actin gene family may be involved in the development of different tissues and response to biotic and abiotic stresses of Charophytes.

关 键 词：布氏轮藻 肌动蛋白基因家族 系统进化分析 组织表达差异 

分 类 号：Q941[生物学—植物学]