基于2b-RAD测序的四倍体马铃薯熟性相关的分子标记开发  

2b-RAD based maturity associated molecular marker identification in tetraploid potato(Solanum tuberosum L.)

作　　者：惠志明 徐建飞[1] 简银巧 卞春松[1] 段绍光[1] 胡军[1] 李广存[1] 金黎平[1] HUI Zhi-Ming;XU Jian-Fei;JIAN Yin-Qiao;BIAN Chun-Song;DUAN Shao-Guang;HU Jun;LI Guang-Cun;JIN Li-Ping(Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences/Key Laboratory of Biology and Genetic Improvement of Tuber and Root Crop,Ministry of Agriculture and Rural Affairs,Beijing 100081,China)

机构地区：[1]中国农业科学院蔬菜花卉研究所/农业农村部薯类作物生物学和遗传育种重点实验室,北京100081

出　　处：《作物学报》2022年第9期2274-2284,共11页Acta Agronomica Sinica

基　　金：宁夏回族自治区农业育种专项马铃薯新品种选育项目(2019NYYZ01-1)资助。

摘　　要：马铃薯熟性是由多基因控制的数量性状,是我国不同栽培区划选择适宜品种的重要指标之一。本研究以晚熟品种中薯18号和早熟品种中薯5号及其F_(1)分离群体为材料,2018—2019年连续2年对“中薯18号(母本)×中薯5号(父本)”杂交分离群体进行熟性评价,从中筛选出极端晚熟和极端早熟的基因型各30个,并分别构建极端晚熟和极端早熟基因组DNA混池。利用简化基因组2b-RAD(2b-restriction site-associated DNA)技术测序,寻找差异标签开发出3个与熟性连锁的分子标记SCARA2-2、SCARA4-21和SCARA5-16,3个分子标记联合使用对熟性分离群体子代进行验证,晚、早熟表型符合率分别到达了87.5%和93.0%,这些分子标记的开发和联合使用对辅助马铃薯熟性选择具有重要的参考价值。Potato maturity is a quantitative trait controlled by multiple genes and it is one of the major agronomic characteristics for selecting suitable varieties in different agro-ecological zones.In this study,the maturity of the segregating population derived from Zhongshu 18(♀)×Zhongshu 5(♂)was evaluated in 2018 and 2019.Respectively,30 offsprings with extremely late or early maturity were selected and used to construct early and late maturing genomic pool.Three molecular markers(SCARA2-2,SCARA4-21,and SCARA5-16)linked to maturity were identified by simplified genome 2b-RAD(2b-restriction site-associated DNA)sequencing.The maturity phenotypic association rate of the three-marker-combination for the late and early maturity geno-type verification reached 87.5%and 93.0%,respectively.Thus,these molecular markers are valuable for markers assisted selec-tion in potato maturity breeding.

关 键 词：马铃薯 熟性 2b-RAD 分子标记 

分 类 号：S532[农业科学—作物学]