Identification of PAL genes related to anthocyanin synthesis in tea plants and its correlation with anthocyanin content  

作　　者：Xuejin Chen Pengjie Wang Mengya Gu Binghao Hou Churan Zhang Yucheng Zheng Yun Sun Shan Jin Naixing Ye 

机构地区：[1]College of Horticulture,Fujian Agriculture and Forestry University/Key Laboratory of Tea Science in University of Fujian Province,Fuzhou 350002,China [2]College of Horticulture,Nanjing Agricultural University,Nanjing 210095,China

出　　处：《Horticultural Plant Journal》2022年第3期381-394,共14页园艺学报（英文版）

基　　金：This research was funded by the Fujian Province“2011 Collaborative Innovation Center”,Chinese Oolong Tea Industry Innovation Center special project(Grant No.J2015-75);China Agriculture Research System of MOF andMARA(GrantNo.CARS-19);Special Fund for Science and Technology Innovation of Fujian Zhang Tianfu Tea Development Foundation(Grant No.FJZTF01).

摘　　要：The phenylalanine ammonia-lyase(PAL)gene family in tea plants(Camellia sinensis L.)encodes the enzyme that catalyzes the first reaction of the phenylpropane metabolic pathway.The present study aimed to characterize the PAL genes in tea plants,and get better insights on the CsPALs in anthocyanins accumulation.Seven CsPAL genes were identified and characterized in tea plants by bioinformatics analysis.Systematic analysis of CsPALs was conducted for its phylogenetic relationship,gene structure,chromosomal location,and protein conserved motifs based on tea plant genome.The cis-elements of CsPALs were responsive to light,abiotic stress,hormone,and MYB-binding site.Furthermore,tissuespecific expression analysis showed that CsPAL4 was expressed preferentially in young leaves and buds.Correlation analysis was performed in purple-leaf tea with anthocyanin components,and it was suggested that CsPAL4 was closely related with different anthocyanin accumulated,especially with cyanidin 3-O-galactoside,cyanidin 3-O-glucoside,and delphinidin 3-O-glucoside.Additionally,the putative upstream regulation factors CsMYBs(CsMYB59,CsARR1,CsSRM1,CsMYB101,and CsMYB52)and CsbHLHs(CsbHLH104,CsbHLH3,CsbIM1,CsTCP14,and CsPIF4)could bind to the promoter of CsPALs,thereby activating its transcription.This study provides a theoretical basis for further research to elucidate the functions of the CsPAL genes.

关 键 词：Camellia sinensis Phenylalanine ammonia-lyase(PAL) Anthocyanin biosynthesis Expression pattern 

分 类 号：S571.1[农业科学—茶叶生产加工]