载Cu-ZnO牙科饰面瓷的制备及抗菌性能  

Preparation and antibacterial properties of Cu⁃ZnO⁃loaded dental veneering porcelain

作　　者：程名扬 乔琴 李群[1] 廖岚[1] CHENG Mingyang;QIAO Qin;LI Qun;LIAO Lan(The Affiliated Stomatology Hospital of Nanchang University,the Key Laboratory of Oral Biomedicine,Jiangxi Province Clinical Research Center for Oral Diseases,Nanchang 330006,China;The Third Hospital of Nanchang,Nanchang 330006,China)

机构地区：[1]南昌大学附属口腔医院,江西省口腔生物医学重点实验室,江西省口腔疾病临床医学研究中心,江西南昌330006 [2]南昌市第三医院,江西南昌330006

出　　处：《口腔疾病防治》2022年第10期705-711,共7页Journal of Prevention and Treatment for Stomatological Diseases

基　　金：国家自然科学基金项目(82160194);江西省自然科学基金项目(20181ACB20022)。

摘　　要：目的 探讨载Cu-ZnO牙科饰面瓷的抗菌性能、生物相容性及机械性能,为研发新型牙科饰面瓷提供实验基础。方法 通过球磨法将纳米Cu-ZnO按照质量百分比0 wt%、1 wt%、2 wt%、3 wt%、4 wt%、5 wt%、6wt%掺杂到用于修复体饰面瓷的IPS E.max Ceram瓷粉中,经高温烧结制备出直径20 mm、厚度2 mm的圆柱形试件。使用扫描电镜观察纳米Cu-ZnO及试件进行表征,观察其表面形貌。采用平板菌落计数法对大肠杆菌(Escherichia coli, E. coli)的抗菌效果进行定量研究。使用CCK-8法评价试件对小鼠成纤维细胞(L929)的体外细胞毒性,并使用荧光显微镜进行细胞活死染色观察。将试件进行三点弯曲强度实验,检测改性后的IPS E.max Ceram饰面瓷的机械性能。结果 扫描电镜下可见块状样结构的Cu-ZnO嵌合于饰面瓷之中。当载纳米Cu-ZnO为1 wt%~4 wt%时试件的抗菌率分别对应为24.85%、67.94%、96.92%、99.99%,0 wt%组与其他浓度组间的差异有统计学意义(F=23.308,P=0.001)。各组与小鼠成纤维细胞(L929)共培养1、3 d后细胞相对增殖率均大于80%,各组间差异无统计学意义,共培养24 h后L929细胞形态正常。试件弯曲强度随Cu-ZnO浓度增加表现为先上升后下降的趋势,载3 wt%纳米Cu-ZnO试件弯曲强度达到最大值(84.72±6.82)MPa,各组间差异无统计学意义(F=0.633,P=0.702)。结论 掺杂3 wt%纳米Cu-ZnO的IPS E.max Ceram饰面瓷经750℃的高温烧结后,对E.coli的抗菌率大于96%,弯曲强度达到最大值(84.728±6.82)MPa,且无明显细胞毒性。Objective To investigate the antibacterial properties,biocompatibility and mechanical properties of Cu-ZnO-loaded dental veneering porcelain to provide an experimental basis for the development of new dental veneering porcelain.Methods Cu-ZnO nanoparticles were added to IPS E.max Ceram for restorative veneer porcelain at different mass percentages of 0 wt%,1 wt%,2 wt%,3 wt%,4 wt%,5 wt%,and 6 wt%using ball milling in ceramic powder.A cylindrical specimen with a diameter of 20 mm and a thickness of 2 mm was prepared by high-temperature sintering.Scanning electron microscopy was used to observe the surface morphologies of nano-Cu-ZnO and the specimens.The antibacterial effect of Escherichia coli(E.coli)was quantitatively studied by the plate colony counting method.The CCK-8 method was used to evaluate in vitro the cytotoxicity of the tested piece to mouse fibroblasts(L929).Live and dead cells were observed by fluorescence microscopy.The mechanical properties of modified IPS E.Max Ceram veneering porcelain were tested by a three-point bending strength test.Results Under the scanning electron microscope,Cu-ZnO appears with a block-like structure and can be seen dispersed in the veneering porcelain.When the nano Cu-ZnO loading was 1 wt%,2 wt%,3 wt%,and 4 wt%,the antibacterial rates of the specimens were 24.85%,67.94%,96.92%,and 99.99%,respectively,and the difference between the experimental groups and the control group was statistically significant(F=23.308,P=0.001).The relative growth rate of each group was greater than 80%after coculture with mouse fibroblast cells(L929)for 1 day and 3 days,and there was no significant difference between the groups.The morphology of L929 cells was normal after coculture for 24 hours.With the increase in the Cu-ZnO concentration,the flexural strength of the specimen exhibited an increasing trend followed by a decreasing trend.The bending strength of the specimen loaded with 3 wt%nano Cu-ZnO reached the maximum value(84.728±6.82)MPa,and there was no statistically significant differenc

关 键 词：纳米铜 纳米氧化锌 纳米材料 纳米铜锌复合抗菌剂 抗菌性能 生物相容性 弯曲强度 热膨胀系数 IPS E.max Ceram饰面瓷 饰面瓷 

分 类 号：R783.1[医药卫生—口腔医学]