多种肿瘤细胞HE4表面岩藻糖基化抗原的表达  

Expressions of fucosylated antigens in human epididymis protein 4 in a variety of cancer cells

作　　者：庄慧宇 张志强[1] 王维 瞿红[1] 娄彤[1] 朱连成[3] 刘娟娟[3] 刘大我[3] 林蓓[3] 王淑珍[1] ZHUANG Huiyu;ZHANG Zhiqiang;WANG Wei;QU Hong;LOU Tong;ZHU Liancheng;LIU Juanjuan;LIU Dawo;LIN Bei;WANG Shuzhen(Department of Obstetrics and Gynecology,Beijing Chao-yang Hospital,Capital Medical University,Beijing 100000,China)

机构地区：[1]首都医科大学附属北京朝阳医院妇产科,北京100000 [2]北京市房山区妇幼保健院妇产科,北京100000 [3]中国医科大学附属盛京医院妇产科,沈阳110004

出　　处：《空军医学杂志》2022年第1期51-54,共4页Medical Journal of Air Force

基　　金：北京市医院管理中心“青苗”计划专项经费资助(QML20210310)。

摘　　要：目的 探讨多种肿瘤细胞(human epididymis protein 4,HE4)表面的岩藻糖基化修饰。方法 分别应用免疫共沉淀、激光共聚焦法检测多种肿瘤细胞中HE4上Lewis y、Lewis x、Lewis a、Lewis b、sLewis a和sLewis x等6种岩藻糖基化抗原的修饰。结果 卵巢癌、肺癌等细胞中的HE4表面均发生了6种岩藻糖基化抗原的修饰,且乳糖系列Ⅱ型糖链的修饰明显高于Ⅰ型糖链。结论 多种肿瘤细胞HE4表面Ⅱ型糖链的修饰,尤其是Lewisy抗原修饰增加有利于肿瘤细胞的侵袭和播散。Objective To investigate human epididymis protein 4(HE4)surface fucosylation in a variety of cancer cells.Methods Co-immunoprecipitation and confocal laser were used to detect six types of fucosylated antigens(Lewis y,Lewis x,Lewis a,Lewis b,sLewis a,and sLewis x)on the human epididymis protein 4(HE4)surface in a variety of cancer cells.Results Six types of fucosylated antigens were identified in cancer cells of the ovary,lung,breast,pancreas and colon.Moreover,the typeⅡsugar chain was more significantly modified than the typeⅠsugar chain of the lactose series.Conclusion The increase of the typeⅡsugar chain of the lactose series,especially Lewis y antigens,has been shown to be conducive to invasion and spread of cancer cells.

关 键 词：人附睾蛋白4 岩藻糖基化抗原 卵巢癌 肺癌 

分 类 号：R73-37[医药卫生—肿瘤]