新疆春小麦新品种(系)高分子量麦谷蛋白优质亚基分析  

Study on the Distribution of HMW-GS in New Breeding(Lines)of Xinjiang Spring Wheat

作　　者：时佳 王重[1,2] 李剑峰 高新[1,2] 张宏芝 王立红[1,3] 王春生 张跃强[1,2] 樊哲儒 陈勋基 SHI Jia;WANG Zhong;LI Jianfeng;GAO xin;ZHANG Hongzhi;WANG Lihong;WANG Cunsheng;ZHANG Yueqiang;FAN Zheru;CHEN Xunji(Institute of Nuclear Technology and Biotechnology, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China;Key Laboratory of Crop Biotechnology of Xinjiang, Urumqi 830091, China;Xinjiang Crop Chemical Control Engineering Technology Research Center, Urumqi 830091, China;Key Laboratory of Desert-Oasis Crop Physiological Ecology and Cultivation, Ministry of Agriculture and Rural Affairs of China, Urumqi 830091, China)

机构地区：[1]新疆农业科学院核技术生物技术研究所,乌鲁木齐830091 [2]新疆农作物生物技术重点实验室,乌鲁木齐830091 [3]新疆作物化学调控工程技术研究中心,乌鲁木齐830091 [4]农业部荒漠绿洲作物生理生态与耕作重点实验室,乌鲁木齐830091

出　　处：《新疆农业科学》2022年第6期1330-1337,共8页Xinjiang Agricultural Sciences

基　　金：国家重点研发计划(2016YFD0102101);新疆农业科学院科技创新重点培育专项(xjkcpy-005);自治区重点实验室开放课题(XJYS0302-2021-3)。

摘　　要：【目的】研究新疆春小麦及CIMMYT引进材料的HMW-GS组成差异,为新疆春小麦杂交选配提供依据。【方法】采用SDS-PAGE电泳技术。分析新疆春小麦192份材料中的HMW-GS的组成及分布频率。【结果】供试材料中共检测出13种HMW-GS等位变异,Glu-A1位点等位变异Null亚基频率最高(64.06%),其次为1亚基(21.35%)和2*亚基(14.58%)。Glu-B1位点等位变异类型表现最丰富,其亚基频率高低顺序为7+8(39.58%)>7(17.19%)>7+9(13.54%)>17+18(11.46%)>22(9.38%)>13+16(7.29%)>6+8(1.04%)>14+15(0.52%);其中7+8、17+18、14+15和13+16亚基为优质亚基,频率为58.85%。Glu-D1位点检测2+12(49.48%)和5+10(50.52%)频率较为接近。在Glu-A1、Glu-B1和Glu-D1位点的优质亚基比例均表现为新疆春小麦品种(系)>引进CIMMYT材料。【结论】全部HMW-GS共形成32种亚基组合,品质评分平均分为7.04分,其中新疆春小麦近年育成的新品种(系)表现出品质评分较高平均分为8.12分,明显高于引进CIMMYT材料品质评分平均为6.09分。评分为10分的材料有25个,评分在8分以上的有90份材料。【Objective】The high molecular weight wheat gluten subunite(HMW-GS)plays an important role in regulating the quality of wheat processing.This project aims to understand the difference in the composition of HMW-GS of Xinjiang spring wheat and introduced CIMMYT materials in the hope of providing a basis for spring wheat hybrid selection in Xinjiang.【Method】SDS-PAGE electrophoresis technology was used to analyze the composition and distribution frequency of HMW-GS in 192 materials of spring wheat in Xinjiang.【Result】The results showed that 13 HMW-GS alleles were detected in the test materials,with the highest Null subunit frequency(64.06%)of the Glu-A1 site allele,followed by 1 subunit(21.35%)and 2 subunit(14.58%).The Glu-B1 site allele variant type was the most abundant,with sub-base frequency order of 7+8(39.58%)>7(17.19%)>7+9(13.54%)>17+18(11.46%)>22(9.38%)>13+16(7.29%)>6+8(1.04%)>14+15(0.52%);The Glu-D1 bit detection frequency of 2 plus 12(49.48%)and 5 plus 10(50.52%)was close.The high-quality sub-base ratio of Glu-A1,Glu-B1 and Glu-D1 sites was shown in the introduction of CIMMYT materials>spring wheat varieties(series)in Xinjiang.【Conclusion】All HMW-GS formed a total of 32 sub-base combinations,the average quality score was divided into 7.04 points,of which the new varieties(departments)of Xinjiang spring wheat cultivated in recent years showed a higher quality score of 8.12 points,significantly higher than the introduction of CIMMYT material quality average score of 6.09 points.There were 25 materials scored at 10 and 90 materials scored above 8.

关 键 词：春小麦 HMW-GS 频率 品质特性 

分 类 号：S512.12[农业科学—作物学]