多数据库研究CRYAB在宫颈癌中的表达及相关机制  

Study of expression and related mechanisms of CRYAB in cervical cancer by using multiple databases

作　　者：黄宏宝 吴小勤 卢东林 覃莉[2] 韦敬锡[2] 罗小琼[2] 黄秋艳 陈发钦[1] Huang Hongbao;Wu Xiaoqin;Lu Donglin;Qin Li;Wei Jingxi;Luo Xiaoqiong;Huang Qiuyan;Chen Faqin(Youjiang Medical University for Nationalities,Baise 533000,Guangxi,China;Center for Reproductive Medicine,The Affiliated Hospital of Youjiang Medical University for Nationalities,Baise 533000,Guangxi,China;Center for Clinicopathological Diagnosis and Research,Baise 533000,Guangxi,China;Department of Gynecology,The Affiliated Hospital of Youjiang Medical University for Nationalities,Baise 533000,Guangxi,China)

机构地区：[1]右江民族医学院,广西百色533000 [2]右江民族医学院附属医院生殖医学中心,广西百色533000 [3]右江民族医学院附属医院临床病理诊断与研究中心,广西百色533000 [4]右江民族医学院附属医院妇科,广西百色533000

出　　处：《右江民族医学院学报》2022年第3期382-388,共7页Journal of Youjiang Medical University for Nationalities

摘　　要：目的利用生物信息学研究αB-晶状体蛋白(CRYAB)在宫颈癌(CC)中的表达情况及其相关机制。方法分别通过UALCAN、临床生信之家和仙桃学术分析CRYAB在宫颈癌及泛癌中的表达;通过GEPIA数据库分析CRYAB与宫颈癌患者生存期的关系;应用UALCAN数据库分析CRYAB与宫颈癌患者临床病理特征的关系;通过TIMER 2.0数据库分析在宫颈癌组织中CRYAB表达量与免疫细胞浸润的相关性;通过LinkedOmics数据库查找CRYAB共表达基因,利用STRING数据库查找关键基因,应用Metascape网站进行GO注释和KEGG通路分析。结果CRYAB在宫颈癌组织中低表达;CRYAB的表达与宫颈癌患者生存期无相关性;其表达量与患者肿瘤分期、人种、体重、年龄、组织学类型和淋巴转移相关;它能诱导机体多种免疫细胞参与肿瘤调控。共表达基因分析显示,按P值升序排列与CRYAB正相关的基因是NDRG4、IVL、KRT75、SBSN和FABP12;负相关的基因是KIAA0649、TMPRSS3、GOLM1、ALDH3B1和RHOBTB2。GO注释显示共表达基因参与表皮发育、包膜角化、多肽交叉连接、钙离子结合和中间纤维细胞骨架组织等,KEGG通路富集在金黄色葡萄球菌感染、粘蛋白O-聚糖生物合成、核苷酸糖的生物合成、IL-17信号通路和癌症的通路等。结论CRYAB在宫颈癌中低表达,与宫颈癌的发生发展存在一定的关系,有望成为宫颈癌的生物标志物和潜在的治疗靶点。Objective To study the expression and related mechanisms ofαB-Crystallin(CRYAB)in cervical cancer(CC)by means of bioinformatics.Methods The expression of CRYAB in cervical cancer and pan-cancer was analyzed by the database UALCAN and the wetsites Assistant for Clinical Bionformatics and Xiantao Academic.The relation between CRYAB and survival of patients with cervical cancer was analyzed with the database GEPIA.The relation between CRYAB and clinicopathological features of patients with cervical cancer was analyzed with the database UALCAN.The correlation of CRYAB expression and immune cell infiltration in cervical cancer tissues was analyzed with the database TIMER 2.0.The co-expressed genes of CRYAB were searched in the database LinkedOmics.Key genes were searched in STRING Database,and GO annotation and KEGG pathway analysis were conducted on the website of Metascape.Results CRYAB was low-expressed in cervical cancer tissues.There was no correlation between CRYAB expression and the survival of cervical cancer patients.Its expression was correlated with the tumor stage,race,weight,age,histological type and lymphatic metastasis of the patient.It could induce various immune cells to participate in tumor regulation.Analysis of co-expressed genes showed that NDRG4,IVL,KRT75,SBSN and FABP12 were positively correlated with CRYAB in the ascending order of P value,while KIAA0649,TMPRSS3,GOLM1,ALDH3B1 and RHOBTB2 were negatively related.GO annotation indicated that co-expressed genes were involed in epidermis development,envelope keratosis,polypeptide cross-linking,calcium ion binding and intermediate fibroskeletal tissues,etc.KEGG pathway was enriched in staphylococcus aureus infection,biosynthesis of mucin o-glycan,biosynthesis of nucleotide sugar,the IL-17 signaling pathway and pathways in cancer.Conclusion CRYAB is low-expressed in cervical cancer,which is in certain degree related with the occurrence and development of cervical cancer.It expected to become a biomarker and potential therapeutic target of cervical c

关 键 词：数据库 生物信息学 αB-晶状体蛋白 宫颈肿瘤 

分 类 号：R737.33[医药卫生—肿瘤]