茶多酚处理脱细胞牛心包组织支架作为人工食管修补材料的可行性  

Feasibility of tea polyphenol-treated bovine pericardial decellularized tissue scaffolds as artificial esophageal repair materials

作　　者：李芹 孙扬永 吴昊 刘晓红 徐志云 Li Qin;Sun Yangyong;Wu Hao;Liu Xiaohong;Xu Zhiyun(Department of Cardiovascular Surgery,Changhai Hospital,Shanghai 200433,China)

机构地区：[1]上海长海医院心血管外科,上海市200433

出　　处：《中国组织工程研究》2023年第12期1832-1836,共5页Chinese Journal of Tissue Engineering Research

基　　金：科技部国家重点研发计划(2016YFC1100900),项目负责人:徐志云。

摘　　要：背景:脱细胞组织支架已被应用于心脏、血管、皮肤、肺部和泌尿科等领域,并逐渐成为食管组织工程的良好候选者。目的:探讨茶多酚处理脱细胞牛心包组织支架作为人工食管材料的可行性。方法:制备脱细胞牛心包组织支架,分别经PBS、戊二醛、茶多酚进行交联处理。将16只SD大鼠随机分4组,A组将经PBS处理的脱细胞牛心包组织支架缝合至食管缺损处,B组将经戊二醛处理的脱细胞牛心包组织支架缝合至食管缺损处,C组将经茶多酚处理的脱细胞牛心包组织支架缝合至食管缺损处,D组暴露食管后关腹,每组4只。术后4周,观察大鼠体质量变化、吻合口情况,修复部位苏木精-伊红染色及肌动蛋白α抗体免疫组化染色。结果与结论:①大体观察可见,A组食管缺损部位肉芽增生,狭窄不明显,部分食管补片材料脱落;B组术后出现吻合口漏1只,食管缺损部位肉芽组织增生明显,大块补片组织突出于食管腔内;C组食管缺损部位肉芽组织增生,缺损部位被人工食管支撑,无明显狭窄;②A、B、C组大鼠术后4周的体质量与D组比较差异无显著性意义(P>0.05);③苏木精-伊红染色显示,A组食管缺损部位肉芽增生,大量炎性细胞浸润,新生食管黏膜部分覆盖补片组织;B组食管缺损部位肉芽组织增生明显,伴有大量炎性细胞浸润,补片周边可见炎性细胞浸润,食管黏膜未覆盖补片组织;C组食管缺损部位肉芽组织增生,炎性细胞浸润较轻,人工食管补片内可见大量新生细胞,原补片组织结构不清,食管黏膜完全覆盖缺损组织;④免疫组化染色显示,A、B组未见肌动蛋白α阳性染色细胞,C组可见蓝染的细胞核,原补片结构疏松,可见肌动蛋白α特异性染色的新生血管组织;⑤结果表明,茶多酚处理脱细胞牛心包组织支架可作为人工食管修复材料。BACKGROUND:Decellularized tissue scaffolds have been used in vascular,cardiovascular,skin,pulmonary,and urology fields,and have gradually become good candidates for esophageal tissue engineering.OBJECTIVE:To investigate the feasibility of tea polyphenol-treated bovine pericardial decellularized tissue scaffolds as artificial esophagus materials.METHODS:The bovine pericardium was decellularized and made into tissue scaffolds,which were treated with PBS,glutaraldehyde or tea polyphenol.Totally 16 SD rats were randomly assigned to four groups(n=4).In group A,PBS-treated decellularized bovine pericardial tissue scaffolds were sutured to esophageal defects.In group B,glutaraldehyde-treated decellularized bovine pericardial tissue scaffolds were sutured to esophageal defects.In group C,tea polyphenol-treated decellularized bovine pericardial tissue scaffolds were sutured to esophageal defects.In group D,the abdomen was sutured after exposing the esophagus.At 4 weeks after the operation,the changes in body weight,anastomotic stoma,hematoxylin-eosin staining and actinαantibody immunohistochemical staining at the repaired site were observed.RESULTS AND CONCLUSION:(1)The general observation showed that in group A,the esophageal defect site had granulation hyperplasia;the stenosis was not obvious;and part of the esophageal patch material fell off.In group B,one patient had anastomotic leakage after operation.The granulation tissue in the esophageal defect site proliferated obviously and the large patch tissue protruded into the esophageal cavity.In group C,granulation tissue hyperplasia was found at the esophageal defect site;the defect site was supported by the artificial esophagus without obvious stenosis.(2)There was no significant difference in body weight between groups A,B and C and group D at 4 weeks after operation(P>0.05).(3)Hematoxylin-eosin staining demonstrated that in group A,granulation hyperplasia was seen at the esophageal defect site;a large number of inflammatory cells infiltrated;and the newly formed eso

关 键 词：人工食管 食管破裂 茶多酚 牛心包 脱细胞组织支架 交联 

分 类 号：R459.9[医药卫生—治疗学] R694[医药卫生—临床医学] R318