可溶性南极磷虾蛋白-芦丁复合物的制备及抗氧化性能分析  

Preparation and antioxidant properties of soluble Antarctic krill protein-rutin complexes

作　　者：李玉锋 王敬敬 檀利军 彭知云 赵晟 刘海泉[2] 赵勇[2] LI Yufeng;WANG Jingjing;TAN Lijun;PENG Zhiyun;ZHAO Cheng;LIU Haiquan;ZHAO Yong(College of Biological and Food Engineering,Anhui Polytechnic University,Wuhu 241000,China;College of Food Sciences and Technology,Shanghai Ocean University,Shanghai 201306,China;Department of Food Science,Foshan University,Foshan 528000,China)

机构地区：[1]安徽工程大学生物与食品工程学院,安徽芜湖241000 [2]上海海洋大学食品学院,上海201306 [3]佛山科学技术学院食品科学与工程学院,广东佛山528000

出　　处：《水产学报》2022年第3期475-485,共11页Journal of Fisheries of China

基　　金：国家重点研发计划(2018YFC1602205);安徽工程大学引进人才科研启动基金(2021YQQ046)。

摘　　要：为了提升可溶性南极磷虾蛋白的抗氧化性能,将芦丁复合到可溶性南极磷虾蛋白(SAKP)上,成功制备了SAKP-芦丁复合物,一种新型的多酚-蛋白复合物。通过测定SAKP-芦丁复合物清除二苯基苦基苯肼自由基(DPPH-)和羟基自由基(OH-)的能力来评估其抗氧化能力。同时采用H_(2)O_(2)诱导HepG-2细胞氧化损伤模型评价了SAKP-芦丁复合物对细胞受到氧化损伤时的保护能力。结果发现,与芦丁复合后,SAKP的抗氧化和清除自由基的能力明显增强;与天然SAKP相比,SAKP-芦丁复合物显著提升了HepG-2细胞超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和谷光甘肽过氧化物酶(GSH-Px)的活性,减少了细胞因氧化应激产生的脂质过氧化产物(MDA)含量,最终降低了氧化应激损伤对细胞的伤害。以上结果表明,SAKP-芦丁复合物是一种新型的、有希望应用于开发功能性食品的蛋白资源。In order to improve the antioxidant activity of Antarctic krill protein,a new polyphenol-protein complex(SAKP-rutin complex)was successfully prepared by grafting rutin onto soluble Antarctic krill proteins(SAKP).The antioxidant capacity of SAKP-rutin complexes was evaluated by measuring its ability to scavenge DPPH-and hydroxyl radicals.Meanwhile,the protective ability of SAKP-rutin complexes against oxidative damage of HepG-2 cells was determined by using an H_(2)O_(2) induced oxidative damage model.The results showed that the combination of rutin greatly enhanced the ability of SAKP to scavenge free radicals.Compared with natural SAKP,the SAKP-rutin complexes significantly increased the activity of cellular antioxidant enzymes(CAT,SOD and GSH-Px),decreased the production of lipid peroxidation products(MDA)in cells caused by oxidative stress,and finally reduced the damage of oxidative stress to cells.These findings suggest that SAKP-rutin complex is a novel protein resource which will hopefully be applied to developing functional protein food.

关 键 词：可溶性南极磷虾蛋白 芦丁 复合物 抗氧化 HEPG-2细胞 

分 类 号：S983[农业科学—捕捞与储运] TS254[农业科学—水产科学]