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作 者:郭琨 王姗姗 刁子洋 陈成[1] 孔得丽 卢淑珺 白志坤[1] 宋铭忻[1] GUO Kun;WANG Shan-shan;DIAO Zi-yang;CHEN Cheng;KONG De-li;LU Shu-jun;BAI Zhi-kun;SONG Ming-xin(Heilongjiang Key Laboratory for Zoonosis/Northe ast Agricultural University,Harbin 150030,China)
机构地区:[1]东北农业大学动物源性人兽共患病黑龙江省重点实验室,黑龙江哈尔滨150030
出 处:《中国兽医科学》2022年第6期736-743,共8页Chinese Veterinary Science
基 金:国家自然科学基金项目(32172883);国家寄生虫资源库项目(NPRC-2019-194-30)。
摘 要:为探究旋毛虫肌幼虫粗提物(Trichinella spiralis muscle larvae crude worm extract,TMCWE)是否干扰旋毛虫寄生的骨骼肌修复过程及其影响保姆细胞形成的分子机制,以C2C12细胞系作为细胞模型,用不同质量浓度的TMCWE刺激C2C12细胞,并设空白对照组,分别于刺激后第0、3、6、12、24小时,通过检测C2C12细胞增殖分化相关因子及信号通路,在体外评估TMCWE对小鼠成肌细胞增殖与分化的影响。结果表明,TMCWE可促进Cyclin D1基因层面和蛋白层面的表达,能抑制分化标志物Myf5、Pax7和MYH及p21基因和蛋白表达水平;TMCWE可以促进AKT及NF-κB的磷酸化,激活PI3K/AKT/NF-κB信号通路。以上研究结果显示,TMCWE可通过上调PI3K/AKT/NF-κB信号通路相关基因表达促进C2C12细胞增殖及下调分化标志分子基因的表达抑制C2C12细胞分化,这为阐明旋毛虫干扰骨骼肌修复和保姆细胞形成的分子机制提供理论依据和基础数据。In order to explore whether the Trichinella spiralis muscle larvae crude worm extract(TMCWE)interferes with the repair process of T.spiralis parasitic skeletal muscle and the molecular mechanism of its influence on the formation of nurse cells,C2C12 cells as cell models were stimulated with different mass concentrations of TMCWE,and blank control groups were set up.At hour 0,3,6,12 and24 after stimulation,the effects of on the proliferation and differentiation of mouse myoblasts were evaluated in vitro by detecting C2C12 cell proliferation and differentiation related factors and signal pathways.The results showed that TMCWE promoted proliferation of C2C12 cells,but inhibited their differentiation,which showed that the expression of cyclin D1 increased and the levels of differentiation markers Myf5,Pax7,MYH and p21 decreased.TMCWE promoted the phosphorylation of AKT and NF-κB,and activated the PI3K/AKT/NF-κB signaling pathway.These results suggested that TMCWE can promote myoblast proliferation and inhibit myoblast differentiation by up-regulating PI3K/AKT/NF-κB signaling pathway.This provided theoretical basis and basic data for clarifying the molecular mechanism of T.spiralis interfering with skeletal muscle repair and nurse cell formation.
分 类 号:S855.9[农业科学—临床兽医学]
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