不同产地库尔勒香梨果实生育期氢、氧稳定同位素特征  

Characteristics of hydrogen and oxygen stable isotopes in different origins Korla fragrant pear fruits in growth period

作　　者：张伟 赵多勇 丁长伟 赵丹 李安[3] 马雪 康露 刘志虎 ZHANG Wei;ZHAO Duo-Yong;DING Chang-Wei;ZHAO Dan;LI An;MA Xue;KANG Lu;LIU Zhi-Hu(College of Food Science and Pharmacy,Xinjiang Agricultural University,Urumqi 830052,China;Institute of Agricultural Quality Standards and Testing Technology,Xinjiang Academy of Agricultural Sciences/Agricultural Product Quality and Safety Risk Assessment Laboratory of Ministry of Agriculture and Rural Affairs/Xinjiang Agricultural Product Quality and Safety Key Laboratory,Urumqi 830091,China;Institute of Quality Standard and Testing Technology,Beijing Academy of Agriculture and Forestry Sciences,Beijing 100097,China;Jiuquan Forest and Fruit Service Center,Jiuquan 735008,China)

机构地区：[1]新疆农业大学食品科学与药学学院,乌鲁木齐830052 [2]新疆农业科学院农业质量标准与检测技术研究所/农业农村部农产品质量安全风险评估实验室/新疆农产品质量安全重点实验室,乌鲁木齐830091 [3]北京市农林科学院质量标准与检测技术研究所,北京100097 [4]酒泉市林果服务中心,酒泉735008

出　　处：《食品安全质量检测学报》2022年第12期4007-4013,共7页Journal of Food Safety and Quality

基　　金：国家自然科学基金项目(31960504、41563002);自治区重点实验室开放课题项目(2019D04013)。

摘　　要：目的分析库尔勒香梨果实生育期氢(H)、氧(O)稳定同位素比值及其变化规律,比较不同产地香梨果实δ^(2)H、δ^(18)O差异。方法以不同产地库尔勒香梨为研究对象,采集花后60~150 d的果实样本,采用真空冷凝提取法抽提果实中的水分,用高温裂解-同位素比值质谱法测定其δ^(2)H、δ^(18)O值,分析香梨生育期H、O同位素特征,通过方差分析法探讨各产地果实水分δ^(2)H、δ^(18)O值的差异。结果新疆库尔勒市、阿克苏市和甘肃酒泉市的香梨生育期果实水δ^(2)H的变化范围分别为‒49.9‰~‒15.4‰、‒62.0‰~‒28.2‰、‒61.5‰~1.5‰,δ^(18)O的变化范围分别为‒2.5‰~6.7‰、‒5.3‰~3.4‰、‒3.5‰~14.2‰,成熟期3个产地δ^(18)O值均存在极显著性差异(P<0.01)。整个生育期3个产地δ^(2)H、δ^(18)O蒸发线均位于当地大气水线右下方,香梨果实水富集作用明显。结论新疆库尔勒市、阿克苏市和甘肃酒泉市香梨果实在整个生育期H、O同位素具有各自的指纹特征,库尔勒的蒸发线斜率和截距更低,果实水蒸发富集^(2)H、^(18)O的能力更强。Objective To analyze the hydrogen(H)and oxygen(O)stable isotope ratios and their changing laws during the growth period of Korla fragrant pear fruits,and compare the differences inδ^(2)H andδ^(18)O of fragrant pear fruits from different origins.Methods Taking the Korla fragrant pear from different origins as the research object,the fruit samples were collected from 60 to 150 days after flowering,and the water in the fruit was extracted by vacuum condensation extraction method.Theδ^(2)H andδ^(18)O values were determined by high temperature isotope-ratio mass spectrometry,and the H and O isotopic characteristics of fragrant pear during the growth period were analyzed,the differences ofδ^(2)H andδ^(18)O values of fruit moisture in different producing areas were analyzed by variance analysis method.Results The variation ranges ofδ^(2)H in fruit water during the growth period of fragrant pears in Korla,Aksu,and Jiuquan,Gansu were‒49.9‰~‒15.4‰,‒62.0‰~‒28.2‰,‒61.5‰~1.5‰,the variation ranges ofδ^(18)O were‒2.5‰~6.7‰,‒5.3‰~3.4‰,‒3.5‰~14.2‰,and there were extremely significant differences inδ^(18)O values in the 3 production areas in the mature stage(P<0.01).During the whole growth period,theδ^(2)H andδ^(18)O evaporation lines of the 3 production areas were located in the lower right of the local atmospheric water line,and the water enrichment effect of fragrant pear fruit was obvious.Conclusion The H and O isotopes of fragrant pear fruits in Korla,Aksu,and Jiuquan,Gansu have their own fingerprint characteristics.During the whole growth period,the slope and intercept of the evaporation line in Korla wre lower,and the ability of fruit water evaporation to enrich ^(2)H and ^(18)O is stronger.

关 键 词：库尔勒香梨 稳定同位素 氢 氧 真实性鉴别 生育期 

分 类 号：S661.2[农业科学—果树学]