miR-21-5p对多发性骨髓瘤细胞增殖、迁移、侵袭及STAT3信号通路的影响  

Effects of miR-21-5p on Proliferation,Migration,Invasion and STAT3 Signaling Pathway of Multiple Myeloma Cells

作　　者：白涛 鲁良 郭晓宁 宫惠琳[4] BAI Tao;LU Liang;GUO Xiao-ning(Department of Orthopedics,Yangling Demonstration Zone Hospital,Yangling Shaanxi 712100)

机构地区：[1]杨凌示范区医院骨科,陕西杨凌712100 [2]杨凌示范区医院脊柱外科,陕西杨凌712100 [3]杨凌示范区医院病理科,陕西杨凌712100 [4]西安交通大学第一附属医院病理科,陕西西安710061

出　　处：《医学临床研究》2022年第5期671-674,共4页Journal of Clinical Research

基　　金：国际科技合作计划项目(2021KW-47)。

摘　　要：【目的】探讨微小RNA(miR)-21-5p对多发性骨髓瘤(MM)细胞增殖、迁移、侵袭及转录激活因子3(STAT3)信号通路的影响。【方法】体外培养人MM RPMI-8226细胞,分为对照组(不进行转染)、siRNA NC组(转染nonsense siRNA)和miR-21-5p siRNA组(转染miR-21-5p siRNA)。实时荧光定量PCR(qRT-PCR)法检测人MM RPMI-8226细胞中miR-21-5p与STAT3表达;CCK-8法检测RPMI-8226细胞增殖;Transwell法检测RPMI-8226细胞迁移、侵袭;蛋白印迹(WB)法检测RPMI-8226细胞中p-STAT3、STAT3、Ki-67、基质金属蛋白酶-9(MMP-9)蛋白表达。【结果】与对照组、siRNA NC组相比,miR-21-5p siRNA组RPMI-8226细胞中miR-21-5p、STAT3 mRNA表达水平、细胞OD值、迁移细胞数、侵袭细胞数、p-STAT3/STAT3、Ki-67、MMP-9蛋白表达水平均显著降低(P<0.05)。【结论】下调miR-21-5p表达可以抑制MM RPMI-8226细胞增殖、迁移及侵袭,并使STAT3信号通路活化受阻。【Objective】To investigate the effects of microRNA(miR)-21-5p on the proliferation,migration,invasion,signal transduction and activator of transcription 3(STAT3)signal pathway of multiple myeloma(MM).【Methods】Human MM RPMI-8226 cells were cultured in vitro and divided into control group(without transfection),siRNA NC group(transfected with nonsense siRNA)and miR-21-5p siRNA group(transfected with miR-21-5p siRNA).The expressions of miR-21-5p and STAT3 in RPMI-8226 cells were detected by real-time fluorescence quantitative PCR(qRT-PCR);The proliferation of RPMI-8226 cells was detected by CCK-8 method;The migration and invasion of RPMI-8226 cells were detected by Transwell method;Western blot(WB)was used to detect the expression of p-STAT3,STAT3,Ki-67 and matrix metalloproteinase-9(MMP-9)in RPMI-8226 cells.【Results】Compared with the control group and the siRNA NC group,the expression levels of miR-21-5p,STAT3 mRNA,cell OD value,number of migrating cells,number of invasive cells,p-STAT3/STAT3,Ki-67 and MMP-9 protein in RPMI-8226 cells in the miR-21-5p siRNA group were significantly lower(P<0.05).【Conclusion】Down-regulating the expression of miR-21-5p can inhibit the proliferation,migration and invasion of MM RPMI-8226 cells,and block the activation of STAT3 signaling pathway.

关 键 词：多发性骨髓瘤 骨髓祖代细胞/细胞学 微RNAS 细胞增殖 

分 类 号：R733.3[医药卫生—肿瘤]