Insights into the mechanism of shortened developmental duration and accelerated weight gain associated with Wolbachia infection in Hylyphantes graminicola  

作　　者：Chunfen LI Meng HE Yinjie CUI Yu PENG Jie LIU Yueli YUN 

机构地区：[1]State Key Laboratory of Biocatalysis and Enzyme Engineering,School of Life Sciences,Hubei University,Wuhan,China

出　　处：《Integrative Zoology》2022年第3期420-429,共10页整合动物学（英文版）

基　　金：This study was financially supported by the National Natural Sciences Foundation of China(NSFC-31573236/31772420/31970406);CAS Key Laboratory of Tropical Forest Ecology,Xishuangbanna Tropical Botanical Garden,Chinese Academy of Sciences(19CAS-TFE-3).

摘　　要：Wolbachia infection is known to affect host reproduction and development.To date,however,the underlying mechanism related to the effects of Wolbachia on host development has not yet been reported.Here,we compared the developmental duration and body weight in different instars of Wolbachia-positive(W+)and Wolbachia-negative(W−)spiders(Hylyphantes graminicola)and detected the relative expression levels of 6 insulin-related genes and 3 ecdysone-related genes using reverse transcription qPCR.Results showed that the developmental duration was significantly shortened in W+spiders compared with W−spiders.Furthermore,W+spiders were significantly heavier thanW−spiders at the 3rd and 4th instars,although no significant differences in body weight were observed after maturity.We also found that the expression levels of insulin-like growth factor-2 mRNA-binding protein-1,insulin-degrading enzyme,and ecdysone-inducible protein-1 genes were significantly down-regulated in W+spiders compared with W−spiders,whereas the expression levels of insulin-like growth factor-binding protein-1,insulin-like peptide receptor,insulin receptor substrate 2-B,insulin-like,ecdysone-induced protein-2,and ecdysone receptor genes were significantly up-regulated inW+spiders.Our results suggest thatWolbachia may influence host development by affecting insulin and ecdysone signaling pathways.

关 键 词：ECDYSONE Hylyphantes graminicola INSULIN WOLBACHIA 

分 类 号：S43[农业科学—农业昆虫与害虫防治]