Characterization of edible bird’s nest by peptide fingerprinting with principal component analysis  

作　　者：Chun-Fai Wong Gallant Kar-Lun Chan Ming-Lu Zhang Ping Yao Huang-Quan Lin Tina Ting-Xia Dong Geng Li Xiao-Ping Lai Karl Wah-Keung Tsim 

机构地区：[1]Division of Life Science and Center for Chinese Medicine R&D,The Hong Kong University of Science and Technology,Clear Water Bay,Kowloon [2]HKUST Shenzhen Research Institute,Hi-Tech Park,Nanshan [3]School of Chinese Herbal Medicine,Guangzhou University of Chinese Medicine [4]Dongguan Mathematical Engineering Academy of Chinese Medicine,Guangzhou University of Chinese Medicine,China

出　　处：《Food Quality and Safety》2017年第1期83-92,共10页食品品质与安全研究（英文版）

基　　金：This research was supported by the grants of Hong Kong Research Grants Council TBS(T13-607/12R),GRF(661110,662911,660411,663012,662713),ITC(UIM/254),TUYF12SC02,TUYF12SC03,TUYF15SC01,The Hong Kong Jockey Club Charities Trust(HKJCCT12SC01);Foundation of The Awareness of Nature(TAON12SC01),SRFDP and RGC ERG Joint Fund(2013009614001/M-HKUST604/13)to Karl Wah-Keung Tsim;National Natural Science Foundation Item(81173498)to Xiao-Ping Lai.

摘　　要：OBJECTIVES:Proteins are the major component and play a key role in nutritious and therapeutic functions of edible bird’s nest(EBN);however,limited studies have been conducted on the protein due to difficulties in extraction,isolation as well as identification.This study aimed to provide comprehensive information for the quality evaluation of EBN peptides,which would be a valuable reference for further study on EBN proteins.METHODS:Here,we developed a quality control method using high performance liquid chromatography(HPLC)peptide fingerprints deriving from EBN being digested with simulated gastric fluid.The characteristic peptide peaks were collected and identified by LC-MS/MS.RESULTS:The characteristic peptide peaks,corresponding to the protein fragments of acidic mammalian chitinase-like,lysyl oxidase,and Mucin-5AC-like,were identified and quantified.Interestingly,the principal component analysis indicated that the fingerprints were able to discriminate colour of EBN(white/red)and production sites(cave/house)of White EBN on the same weight basis.As proposed by the model developed in this study,Muc-5AC-like and AMCaselike proteins were the markers with the highest discriminative power.CONCLUSIONS:The overall findings suggest that HPLC peptide fingerprints were able to clearly demonstrate peptide profile differences between genuine and adulterated EBN samples;and classify EBN samples by its color and production site.In addition,the protein identification results suggested that Muc-5AC-like protein was the major protein in EBN.

关 键 词：Edible bird’s nest Peptide fingerprint Mucin-5AC-like Acidic mammalian chitinase-like Principal component analysis 

分 类 号：R47[医药卫生—护理学]