Role of transient receptor potential vanilloid-1 in behavioral thermoregulation of the Mongolian gerbil Meriones unguiculatus  

作　　者：Jing WEN Tingbei BO Zhijun ZHAO Dehua WANG 

机构地区：[1]State Key Laboratory of Integrated Management of Pest Insects and Rodents,Institute of Zoology,Chinese Academy of Sciences,Beijing,China [2]School of Life and Environmental Sciences,Wenzhou University,Wenzhou,China [3]CAS Center for Excellence in Biotic Interactions,University of Chinese Academy of Sciences,Beijing,China [4]School of Life Sciences,Shandong University,Qingdao 266237,China

出　　处：《Integrative Zoology》2022年第4期608-618,共11页整合动物学（英文版）

基　　金：This work was supported by the National Natural Science Foundation of China(No.31970417 and 31772461)to DHW;the State Key Laboratory of Integrated Management of Pest Insects and Rodents(Grant No.IPM2004).

摘　　要：Ambient temperature considerably affects the physiology and behavior of mammals.Thermosensory and thermoregulatory abilities play an important role in the response to changing ambient temperature in endotherms.However,the molecular mechanisms of behavioral thermoregulation remain poorly understood.Transient receptor potential vanilloid-1(TRPV1)is activated by changes in ambient temperature and is involved in acute thermoregulation.Here,we aimed to determine whether TRPV1 is involved in behavioral thermoregulation in wild rodents by conducting 2 experiments.In the first,42 adult Mongolian gerbils(Meriones unguiculatus;14 per treatment)were randomly assigned to 3 housing temperatures(4,23,and 36℃for 4 weeks.In the second,20 gerbils(10 per treatment)were randomly injected with capsaicin(TRPV1 agonist)or AMG517(TRPV1 antagonist).The results showed a significant decrease in food intake and non-shivering thermogenesis in the gerbils housed at 36℃.Additionally,there was a significant increase in the preference of gerbils housed at 4℃ to low temperatures.The expression of TRPV1 protein in the brown adipose tissue(BAT)and liver was significantly positively correlated with that of protein kinase A(PKA).The expression of TRPV1 and PKA proteins in the BAT was positively correlated with the temperature preference of the gerbils.The gerbils injected with capsaicin preferred significantly lower temperatures than the control group gerbils.These findings suggest that TRPV1 and PKA are involved in behavioral thermoregulation in Mongolian gerbils.

关 键 词：brown adipose tissue(BAT) CAPSAICIN Mongolian gerbils protein kinase A(PKA) transient receptor potential vanilloid-1(TRPV1) 

分 类 号：Q95-33[生物学—动物学]