燕麦青稞粉对Ⅱ型糖尿病小鼠糖脂代谢及体内氧化应激的影响  

Effect of Oat Barley Powder on Glucose and Lipid Metabolism and Oxidative Stress in TypeⅡDiabetic Mice

作　　者：高晓晗 王利国 张诗文 戴雪伶[1] 张静[1] 魏涛[1] 米生权[1] 孙雅煊[1] Gao Xiaohan;Wang Liguo;Zhang Shiwen;Dai Xueling;Zhang Jing;Wei Tao;Mi Shengquan;Sun Yaxuan(Beijing Key Laboratory of Bioactive Substances and Functional Foods,Beijing Union University,Beijing 100191;Guzhen Zhangjiakou Food Co.,Ltd.,Zhangjiakou 075000,Hebei;Beijing Daxing Xinghua High School,Beijing 102600)

机构地区：[1]北京联合大学生物活性物质与功能食品北京市重点实验室,北京100191 [2]谷之禅张家口食品有限公司,河北张家口075000 [3]北京大兴兴华中学,北京102600

出　　处：《中国食品学报》2022年第6期190-197,共8页Journal of Chinese Institute Of Food Science and Technology

摘　　要：目的:探讨燕麦青稞粉对Ⅱ型糖尿病小鼠糖脂代谢及体内氧化应激的影响。方法:采用高脂饲料喂养ICR小鼠,腹腔注射小剂量链脲佐菌素(50 mg/kg)建立糖尿病小鼠模型。将成模小鼠随机分为3组,分别为高脂组、阳性对照组【二甲双胍200 mg/(kg·d)】和高脂燕麦青稞粉组。喂养6周期间,观察每组的体重和血糖水平变化,6周后摘眼球取血,检测血脂、空腹血糖及血清胰岛素。留取肝脏组织测定丙二醛(MDA)含量、超氧化物歧化酶(SOD)活力、谷胱甘肽过氧化物酶(GSH-Px)活力以及谷胱甘肽(GSH)等氧化应激标志物水平。结果:与高脂组比较,高脂燕麦青稞粉组能有效缓解糖尿病小鼠的症状,HE染色表明,燕麦青稞粉可以改善小鼠胰腺组织病理状态;Ⅱ型糖尿病小鼠空腹血糖水平显著降低(P<0.05),HDL-c和LDL-c含量极显著降低(P<0.01),TG含量无显著变化(P>0.05),TC含量显著降低(P<0.05);GSH-px水平得到显著升高(P<0.05),SOD的水平显著升高(P<0.05),MDA含量无显著差异(P>0.05)。与高脂组相比较,高脂燕麦青稞粉组血清胰岛素极显著升高(P<0.01),高脂组与空白对照组比较,极显著升高(P<0.01)。喂养燕麦青稞粉的高脂组小鼠中肠道菌群的乳酸菌含量与高脂组相比得到改善。结论:燕麦青稞粉对Ⅱ型糖尿病小鼠血糖水平有降低作用,可在一定程度上调节血脂代谢紊乱,其改善Ⅱ型糖尿病的具体机制可能与提高机体抗氧化活性相关。Objective:To investigate the effects of oat barley powder on glucose and lipid metabolism and oxidative stress in typeⅡdiabetic mice.Methods:ICR mice were fed high-fat diet,combined with small dose(50 mg/kg)intraperitoneal injection of streptozotocin to establish a diabetic mouse model.The adult mice were randomly divided into a high-fat group,a positive control group(metformin 200 mg/kg·d)and a high-fat oat barley powder group.Observe the changes in body weight and blood glucose of each group during the sample administration,take the blood from the eyeball after 6 weeks of sample administration,and detect blood lipids,fasting blood sugar and serum insulin.The liver tissue was collected to determine the activity of malonaldehyde(MDA),superoxide dismutase(SOD),glutathione peroxidase(GSH-Px)activity,and glutathione(GSH).Results:Compared with the high-fat group,the high-fat oat barley powder group can effectively alleviate the symptoms of diabetic mice.HE staining showed that oat barley powder improved the pathological state of the mouse pancreas;it could significantly reduce the fasting blood glucose level of typeⅡdiabetic mice(P<0.05),it has a very significant effect on reducing HDL-c and LDL-c content(P<0.01),there is no significant difference in TG content(P>0.05),and it has a significant effect on TC reduction(P<0.05);The level of GSH-px was significantly increased(P<0.05),the level of SOD was significantly increased(P<0.05),and there was no significant difference in the content of MDA(P>0.05).Compared with the high-fat group,the high-fat oat barley powder group had a very significant increase in serum insulin(P<0.01),and the high-fat group had a very significant increase(P<0.01)compared with the blank control group.The content of lactic acid bacteria in the intestinal flora in the high-fat group fed oat barley meal was improved compared with the high-fat group.Conclusion:Oat barley powder has the effect of lowering blood sugar in typeⅡdiabetic mice,and has a certain regulatory effect on the disorder of

关 键 词：燕麦青稞粉 Ⅱ型糖尿病 血糖 血脂 氧化应激 

分 类 号：TS210.1[轻工技术与工程—粮食、油脂及植物蛋白工程]