原纤维蛋白1基因在胃癌组织中的表达、功能富集及信号通路生物信息学分析  

Bioinformatics analysis of the expression,functional enrichment and signal pathway of fibrillin 1 gene in gastric cancer

作　　者：姚坤厚[1] 李昊 王晨宇[1] 张军杰[1] 李兴旺 YAO Kunhou;LI Hao;WANG Chenyu;ZHANG Junjie;LI Xingwang(Department of General Surgery,Huaihe Hospital,Henan University,Kaifeng 475000,Henan Province,China)

机构地区：[1]河南大学淮河医院普通外科,河南开封475000

出　　处：《新乡医学院学报》2022年第7期669-672,共4页Journal of Xinxiang Medical University

基　　金：河南省科技厅科技公关计划项目(编号:182102310372,192102310091)。

摘　　要：目的探讨原纤维蛋白1(FBN1)基因在胃癌组织中的表达、相关生物学功能和信号通路以及FBN1表达与胃癌患者生存期的关系。方法应用生物信息大数据挖掘技术分析癌症基因组图谱数据库(TCGA)中人胃癌癌组织和癌旁正常组织中FBN1 mRNA表达水平。应用蛋白相互作用数据库STRING构建FBN1基因相关蛋白-蛋白相互作用(PPI)网络并对蛋白网络中的编码基因进行功能富集和京都基因与基因组百科全书(KEGG)信号通路分析。根据胃癌组织中FBN1 mRNA表达水平的中位数将胃癌患者分为高表达组(n=126)和低表达组(n=118),采用Cox回归模型比较FBN1高表达组与低表达组患者总生存期(OS)和无疾病进展生存期(DFS)。结果FBN1 mRNA在胃癌癌组织中的表达显著高于癌旁正常组织(P<0.05)。FBN1基因PPI网络中共有21个蛋白和143个相互作用关系,各蛋白平均相互作用指数为0.816,网络中各个蛋白功能富集明显(P<0.05)。FBN1基因生物学过程主要富集于翻译后蛋白质修饰、解剖结构发育等;细胞组分主要富集于内质网内腔、细胞外基质和内质网等;分子功能主要富集于细胞黏附分子结合和整合素结合等;KEGG分析显示,FBN1基因KEGG主要富集于细胞外基质受体相互作用、黏着及胃肠道肿瘤等。FBN1 mRNA表达与CD8^(+)T细胞、CD4^(+)T细胞、巨噬细胞、中性粒细胞及树突状细胞浸润呈正相关(r=0.248、0.412、0.665、0.353、0.412,P<0.05)。FBN1高表达组患者的OS和DFS均显著短于低表达组(P<0.05)。结论FBN1基因在胃癌组织中表达水平显著升高,且高表达患者的OS和DFS显著短于低表达患者。FBN1基因编码蛋白功能富集明显。Objective To investigate the expression,related biological function and signal pathway of fibrillin 1(FBN1)gene in gastric cancer tissue,and the relationship between the FBN1 expression and survival time of gastric cancer patients.Methods The expression of FBN1 gene in cancer tissue and adjacent normal tissue of gastric cancer was analyzed through the Cancer Genome Atlas Database(TCGA).FBN1 gene-related protein-protein interaction(PPI)network was constructed by protein interaction database STRING,and the functional enrichment and Kyoto Encyclopedia of Genes and Genomes(KEGG)signal pathway analysis of coding genes in PPI were performed.The gastric cancer patients were divided into high expression group(n=126)and low expression group(n=118)according to the median FBN1 mRNA expression level in gastric cancer tissue,and the overall survival(OS)and progression free survival(DFS)of patients were compared between the high expression group and the low expression group by Cox regression model.Results The expression of FBN1 mRNA in gastric cancer tissue was significantly higher than that in adjacent normal tissue(P<0.05).There were 21 proteins and 143 interactions in the PPI network encoded by FBN1 mRNA;the average interaction index of each protein was 0.816,and the fanction enrichment of each protein in the network was obvious(P<0.05).The biological process of FBN1 gene was mainly enriched in the post-translational protein modification and anatomical structure development,etc;cell components were mainly enriched in the endoplasmic reticulum lumen,extracellular matrix and endoplasmic reticulum,etc;molecular functions were mainly enriched in the structural components of cell adhesion molecule binding and integrin binding,etc;KEGG analysis results showed that the FBN1 gene was mainly enriched in the extracellular matrix receptor interaction,adhesion and gastrointestinal tumor.The expression of FBN1 mRNA was positively correlated with the infiltration of CD8^(+)T cells,CD4^(+)T cells,macrophages,neutrophils and dendritic cell

关 键 词：胃癌 原纤维蛋白1 信号通路 

分 类 号：R735.2[医药卫生—肿瘤]