苎麻种质镉积累量与EST-SSR的关联分析  

Association Analysis of Cadmium Accumulation with EST-SSR in Ramie

作　　者：张英[1,2] 许岳军 汪娅梅 曾怡 马玉申 邢虎成[1,2] 揭雨成 Zhang Ying;Xu Yuejun;Wang Yamei;Zeng Yi;Ma Yushen;Xing Hucheng;Jie Yucheng(Institute of Ramie,Hunan Agricultural University,Changsha,410128;Hunan Engineering Technology Center of Grass Crop Germplasm Innovation and Utilization,Changsha,410128)

机构地区：[1]湖南农业大学苎麻研究所,长沙410128 [2]湖南省草类作物种质创新与利用工程技术研究中心,长沙410128

出　　处：《分子植物育种》2022年第12期4004-4018,共15页Molecular Plant Breeding

基　　金：国家科技平台项目(NICGR2018-072);国家自然科学基金项目(31371704;31872877)共同资助。

摘　　要：为进一步加快苎麻高镉累积能力优良品种的选育过程,本研究利用转录组数据开发的168对具有良好多态性的EST-SSR引物与269份苎麻种质的地上部、地下部和单蔸镉积累量进行关联分析,以期得到与镉积累量显著关联的位点。研究结果显示:在显著水平P<0.05条件下,通过广义线性模型(GLM)检测到1个标记(22169-2)在不同年份间与地上部镉积累量显著关联,5个标记(15123,4849,6131,13634和10753)在不同年份间与地下部镉积累量均显著关联,2个标记(6131和13634)在不同年份间与单蔸镉积累量均显著关联。本研究关联得到的EST-SSR标记可为进一步探究苎麻重金属镉胁迫耐受和富集机理及其遗传育种等方面提供理论依据。In order to accelerate the breeding process of ramie with high cadmium accumulation ability,the study used 269 ramie germplasm resources in our experiments.We used a total of 168 pairs EST-SSR primers to obtain the loci,which associated with cadmium accumulation,significantly.The results showed that 1 marker(22169-2)was significantly correlated with the aboveground cadmium accumulation.5 markers(15123,4849,6131,13634and 10753)were significantly correlated with the underground cadmium accumulation,and 2 markers(6131 and13634)were significantly correlated with total cadmium accumulation.The EST-SSR markers obtained in this study can provide a theoretical basis for the further exploring the mechanism of cadmium stress and its genetics and breeding in ramie.

关 键 词：苎麻(Boehmeria nivea) 镉积累量 EST-SSR 标记 

分 类 号：S563.1[农业科学—作物学]