野生白棕口蘑菌种分离及培养条件优化  

Optimization of Isolation and Culture Conditions of Wild Edible Fungus-Tricholoma albobrunneum

作　　者：陆欢[1] 王瑞娟[1] 万佳宁[1] 杨慧[1] 徐珍[1] 尚晓冬[1] 刘建雨 Lu Huan;Wang Ruijuan;Wan Jianing;Yang Hui;Xu Zhen;Shang Xiaodong;Liu Jianyu(Key Laboratory of Agricultural Genetics and Breeding of Shanghai,National R&D Center for Edible Fungi Processing,National Engineering Research Center of Edible Fungi,Key Laboratory of Edible Fungi Resources and Utilization(South),Ministry of Agriculture and Rural Affairs,P.R.China,Institute of Edible Fungi,Shanghai Academy of Agricultural Sciences,Shanghai,201403)

机构地区：[1]上海市农业科学院食用菌研究所,农业部应用真菌资源与利用重点开放实验室,国家食用菌工程技术研究中心,上海市农业遗传育种重点实验室,上海201403

出　　处：《分子植物育种》2022年第12期4019-4027,共9页Molecular Plant Breeding

基　　金：上海市农业科学院卓越团队建设计划项目(ZY1601)资助。

摘　　要：本研究对从江西赣州采集的一株野生食用菌子实体经组织分离获得菌丝纯培养物,通过形态特征分析和ITS技术进行鉴定,鉴定其为口蘑属的白棕口蘑Tricholoma albobrunneum。序列上传GenBank,登录号为MT539126。本研究还通过不同培养基配方对白棕口蘑进行分离纯化培养,并设计分析不同碳源和氮源培养基对白棕口蘑菌丝生长的影响。结果表明加入菌根泥土浸提液的4号培养基菌丝的生长速度最快,菌丝长势强,色素分泌少,得到纯化菌种。白棕口蘑菌丝生长最佳碳源为红糖,最佳氮源为酵母膏,最佳培养基组合为:马铃薯200 g,红糖20 g,酵母膏5 g,琼脂20 g,KH_(2)PO_(4)0.6 g,MgSO_(4)0.6 g,pH自然。本研究为后续白棕口蘑栽培试验提供了一定理论基础。A wild edible fungus fruiting body was collected from Ganzhou,Jiangxi Province and was isolated,identified by morphological and molecular characterization.The wild edible fungus was identified as Tricholoma albobrunneum of Tricholoma.Its ITS sequence submission to NCBI obtained GenBank number MT539126.Different medium formulations were used for isolation and purification of Tricholoma albobrunneum,and analyzed the effects of different carbon and nitrogen sources on the growth rate of Tricholoma albobrunneum.The results showed that the mycorrhizal soil extract was added to the medium 4,the mycelium growth rate was the fastest,the mycelium growth was strong,the pigment secretion was less.The best carbon source was brown sugar,the best nitrogen source was yeast extract,and the best medium combination was potato 200 g,brown sugar 20 g,yeast extract 5 g,agar 20 g,KH_(2)PO_(4)0.6 g,MgSO_(4)0.6 g,pH natural.It provided a basis for the later cultivation of Tricholoma albobrunneum.

关 键 词：白棕口蘑 分离纯化 碳源 氮源 生长速度 

分 类 号：S646[农业科学—蔬菜学]