冷应激对科尔沁牛与科尔沁牛杂交牛的生理指标及抗氧化功能的影响  

Effect of cold stress on physiological indexes and antioxidant function of Kerqin cattle and crossbred Kerqin cattle

作　　者：包雨鑫 吴佳琦 王梓 高丽娟 郭煜 郑海英 付明山 韩玉国 BAO Yuxin;WU Jiaqi;WANG Zi;GAO Lijuan;GUO Yu;ZHENG Haiying;FU Mingshan;HAN Yuguo(Tongliao Agriculture and Animal Husbandry Research Institute,Tongliao 028000,China)

机构地区：[1]通辽市农牧科学研究所,内蒙古通辽028000

出　　处：《黑龙江畜牧兽医》2022年第8期51-54,共4页Heilongjiang Animal Science And veterinary Medicine

基　　金：内蒙古自治区2018年科技重大专项“科尔沁肉牛品种培育”(2060901)。

摘　　要：为研究冷应激对科尔沁牛与科尔沁牛杂交牛生理指标及抗氧化功能的影响,试验选取了身高、体重、胎次相近的健康科尔沁牛与科尔沁牛杂交牛母牛各20头为研究对象,分冷应激期(2019年12月15—21日)和非冷应激期(2020年4月11—17日)两个阶段进行试验,在试验第7天测定两个品种牛的呼吸频率与直肠温度,以及血清超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)活性、丙二醛(MDA)含量及总抗氧化能力(T-AOC),分析在非冷应激和冷应激条件下两品种牛的呼吸频率、直肠温度及抗氧化功能差异。结果表明:两个品种牛在冷应激期内的呼吸频率、直肠温度均显著低于非冷应激期(P<0.05);两个品种牛在非冷应激期内血清SOD活性均极显著高于冷应激期(P<0.01),MDA含量均极显著低于冷应激期(P<0.01),T-AOC与冷应激期差异不显著(P>0.05);科尔沁牛杂交牛非冷应激期内血清GSH-Px活性与冷应激期差异不显著(P>0.05),但冷应激期科尔沁牛的GSH-Px活性比非冷应激期极显著降低(P<0.01)。在非冷应激期时,两个品种牛的呼吸频率、直肠温度及血清SOD和GSH-Px活性、MDA含量及T-AOC没有显著差异(P>0.05)。在冷应激期时,与科尔沁牛比,科尔沁牛杂交牛的直肠温度显著提高(P<0.05),呼吸频率变化不显著(P>0.05),血清SOD活性显著提高(P<0.05),GSH-Px活性极显著提高(P<0.01),MDA含量极显著降低(P<0.01),T-AOC有升高趋势(P>0.05)。说明冷应激对不同品种牛均造成了影响,但科尔沁牛杂交牛的体温调节功能和抗氧化功能更佳,更能够适应内蒙古高寒环境。In order to study the effect of cold stress on the physiological indexes and antioxidant function between Kerqin cattle and crossbreed Kerqin cattle, 20 healthy Kerqin cattle and Kerqin crossbred cattle with similar height, weight and parity were selected as research objects. The experiment was divided into two phases: cold stress(December 15-21,2019) and non-cold stress(April 11-17,2020). Respiration rate, rectal temperature, the activity of superoxide dismutase(SOD) and glutathione peroxidase(GSH-Px), malondialdehyde(MDA) content and total antioxidant capacity(T-AOC) in two breeds of cattle were determined on the 7 th day of the experiment. The difference of respiratory rate, rectal temperature and antioxidant capacity in two breeds of cattle was analyzed under cold stress and non-cold stress. The experiment results were as follow: the respiratory rate and rectal temperature in cold stress period were significantly lower than those in non-cold stress period(P<0.05);in non-cold stress period, SOD activity in the serum was significantly higher than that in cold stress period(P<0.01), and the content of MDA was significantly lower than that in cold stress period(P<0.01). There was no significant difference in T-AOC between non-cold stress period and cold stress period(P>0.05). There was no significant difference in serum GSH-Px activity of crossbred Kerqin cattle between non-cold stress period and cold stress period(P>0.05), but the serum GSH-Px activity of Kerqin cattle in the cold stress period was significantly lower than that in the non-cold stress period(P<0.01). Under non-cold stress, there was no significant difference in respiration rate, rectal temperature, the activities of SOD and GSH-Px, MDA content and T-AOC in two breeds of cattle. Under cold stress, rectal temperature of crossbred Kerqin cattle was significantly higher than that of Kerqin cattle(P<0.05);the respiration rate was not significantly changed(P>0.05);serum SOD activity was significantly increased(P< 0.05);GSH-Px activity was significantly

关 键 词：冷应激 科尔沁牛杂交牛 科尔沁牛 抗氧化功能 生理指标 

分 类 号：S823.8[农业科学—畜牧学]