Multidrug-resistant Proteus mirabilis isolates carrying blaOXA-1 and blaNDM-1 from wildlife in China:increasing public health risk  

作　　者：Qian KANG Xue WANG Jianan ZHAO Zhihui LIU Fang JI Han CHANG Jianchun YANG Shijia HU Ting JIA Xiaojia WANG Jiagui TANG Guoying DONG Guocheng HU Jing WANG Yanyu ZHANG Jianhua QIN Chengmin WANG 

机构地区：[1]Guangdong Key Laboratory of Animal Conservation and Resource Utilization,Guangdong Public Laboratory of Wild Animal Conservation and Utilization,Institute of Zoology,Guangdong Academy of Science,Guangzhou,Guangdong,China [2]College of Veterinary Medicine,Agricultural University of Hebei,Baoding,Hebei,China [3]Institute of zoology,Chinese Academy of Sciences,Beijing,China [4]Beijing Key Laboratory of Captive Wildlife Technologies,Beijing Zoo,Beijing,China [5]Chongqing Zoo,Chongqing [6]College of Global Change and Earth System Science,Beijing Normal University,Beijing,China [7]South China Institute of Environmental Sciences,Ministry of Ecology and Environment,Guangzhou,Guangdong,China [8]Department of Infectious Diseases,Hangzhou Center of Disease Control and Prevention,Zhejiang,China [9]Beijing Key Laboratory of Blood Safety and Supply Technologies,Institute of Health Service and Transfusion Medicine,Beijing,China

出　　处：《Integrative Zoology》2021年第6期798-809,共12页整合动物学（英文版）

基　　金：This study was supported by the Introduction of Leading Talents Program of Guangdong Academy of Sciences(No.2016GDASRC-0205);GDAS Special Project of Science and Technology Development(No.2018GDASCX-0107);Earmarked Fund for Hebei Dairy Cattle Innovation Team of Modern Agro-industry Technology Research System(No.HBCT2018120205).

摘　　要：The emergence of multidrug resistance(MDR)in Proteus mirabilis clinical isolates is a growing public health concern and has serious implications for wildlife.What is the role of wildlife has been become one of the hot issues in disseminating antimicrobial resistance.Here,54 P.mirabilis isolates from 12 different species were identified.Among them,25 isolates were determined to be MDR by profile of antimicrobial susceptibility;10 MDR P.mirabilis isolates were subjected to comparative genomic analysis by whole genome sequencing.Comprehensive analysis showed that chromosome of P.mirabilis isolates mainly carries multidrug-resistance complex elements harboring resistance to carbapenem genes blaOXA-1,blaNDM-1,and blaTEM-1.Class I integron is the insertion hotspot of IS26;it can be inserted into type I integron at different sites,thus forming a variety of multiple drug resistance decision sites.At the same time,Tn21,Tn7,and SXT/R391 mobile elements cause widespread spread of these drug resistance genes.In conclusion,P.mirabilis isolates from wildlife showed higher resistance to commonly used clinic drugs comparing to those from human.Therefore,wild animals carrying MDR clinical isolates should be paid attention to by the public health.

关 键 词：blaNDM-1 blaOXA-1 MULTIDRUG-RESISTANT Proteus mirabilis WILDLIFE 

分 类 号：S855.3[农业科学—临床兽医学]