特定浓度甘露醇对SPC-A-1人肺癌细胞的凋亡实验研究  

Basic study on the antitumor effect of mannitol at specific concentration

作　　者：李黄羿[1] 黄鼎铭[1] Li Huangyi;Huang Dingming(.The Affiliated Cancer Hospital of Guangxi Medical University,Nanning,Guangxi 530021)

机构地区：[1]广西医科大学附属肿瘤医院,广西南宁530021

出　　处：《基层医学论坛》2022年第22期1-3,7,共4页The Medical Forum

基　　金：2022年广西教育厅中青年科研基础能力提升项目(2022KY0106)。

摘　　要：目的探讨特定浓度甘露醇是否有诱导SPC-A-1人肺癌细胞凋亡的作用。方法体外培养的肺癌细胞,经不同浓度的甘露醇处理后分别采用MTT法、化学发光技术和流式细胞技术,检测细胞增殖水平以及甘露醇对细胞的抑制率。结果甘露醇在120μg/μl~140μg/μl浓度(即12%~14%甘露醇)48小时内即可达到理想抑制作用,且作用稳定。结论特定浓度的甘露醇可以诱导SPC-A-1人肺癌细胞凋亡,其作用强度与时间和不同浓度有关。Objective To investigate whether specific concentration of mannitol can induce apoptosis of human SPC-A-1 lung cancer cells.Methods MTT assay,chemiluminescence assay and flow cytometry were used to detect the proliferation level of lung cancer cells cultured in vitro and the inhibition rate of mannitol.Results The ideal inhibitory effect of mannitol was achieved within 48 hours at the concentration of 120μg/μl~140μg/μl(i.e.12%~14%mannitol),and the effect was stable.Conclusion The specific concentration of mannitol can induce apoptosis of human SPC-A-1 lung cancer cells,and the effect intensity is related to time and different concentration.

关 键 词：肺癌 细胞凋亡 甘露醇 MTT比色法 化学发光法 流式细胞技术 

分 类 号：R73-36[医药卫生—肿瘤]