含铁离子纳米络合物的制备及其诱导肿瘤细胞铁死亡作用  

Preparation of iron-containing nanocomplex and its inducing ferroptosis in tumor cells

作　　者：张潇童 周凯悦 徐文男 徐强[3] 尹浩东 郭修晗[1,4] ZHANG Xiaotong;ZHOU Kaiyue;XU Wennan;XU Qiang;YIN Haodong;GUO Xiuhan(Department of Pharmacy,School of Chemical Engineering,Dalian University of Technology,Dalian 116024,Liaoning,China;School of Chemistry and Chemical Engineering,Hulunbuir University,Hulunbuir 021008,Inner Mongolia,China;School of Analysis and Testing Center,Dalian University of Technology,Dalian 116024,Liaoning,China;School ofNingbo Research Institute,Dalian University of Technology,Ningbo 315016,Zhejiang,China)

机构地区：[1]大连理工大学化工学院药学系,辽宁大连116024 [2]呼伦贝尔学院化学与化工学院,内蒙古呼伦贝尔021008 [3]大连理工大学分析测试中心,辽宁大连116024 [4]大连理工大学宁波研究院,浙江宁波315016

出　　处：《精细化工》2022年第6期1218-1222,共5页Fine Chemicals

基　　金：国家自然科学基金(21506024);教育部留学回国人员科研启动基金(No.49);中央高校基本科研业务费(DUT20LK08)。

摘　　要：铁死亡是一种新发现的程序性细胞死亡过程,与铁依赖的脂质过氧化代谢有关。以表没食子儿茶素没食子酸酯(EGCG)和Fe^(3+)配合形成的Fe^(Ⅲ)EGCG网络结构为纳米载体,负载铁死亡诱导剂索拉菲尼(SRF),制备了SRF@Fe^(Ⅲ)EGCG纳米络合物。对其进行了粒径、Zeta电位及各物质的质量分数的测定,并测试了SRF@Fe^(Ⅲ)EGCG纳米络合物中SRF的包封率和载药量。体外细胞实验表明,SRF@Fe^(Ⅲ)EGCG纳米络合物对Hela细胞的生长抑制作用明显,细胞存活率约为30%。此外,发现SRF@Fe^(Ⅲ)EGCG纳米络合物存在多种形式的肿瘤细胞生长抑制相协同的效果,诱导细胞铁死亡确实是其作用途径之一。Ferroptosis is a newly discovered cell death process related to iron-dependent lipid peroxidation metabolism.The coordination of epigallocatechin-3-gallate(EGCG)and Fe^(3+)formed Fe^(Ⅲ)EGCG network structure.Then,ferroptosis inducer sorafenib(SRF)was loaded on Fe^(Ⅲ)EGCG network structure to prepare SRF@Fe^(Ⅲ)EGCG nanocomplex.The particle size and Zeta potential of SRF@Fe^(Ⅲ)EGCG nanocomplex and the mass fraction of each substance in SRF@Fe^(Ⅲ)EGCG nanocomplex were tested.The encapsulation rate and drug loading of SRF in SRF@Fe^(Ⅲ)EGCG nanocomplex were measured.Cell tests in vitro showed that SRF@Fe^(Ⅲ)EGCG nanocomplex had obvious inhibitory effect on the growth of Hela tumor cells,and the survival rate of tumor cells was about 30%.In addition,it was found that the nanocomplex had a synergistic effect on tumor cell growth inhibition in various forms,and induction of cell ferroptosis was indeed one of the ways of action of the nanocomplex.

关 键 词：金属-多酚网络 索拉菲尼 表没食子儿茶素没食子酸酯 铁死亡 医药原料 

分 类 号：R943[医药卫生—药剂学] R965[医药卫生—药学]